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NA02A

Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Agarose Conjugate

Name: Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Agarose Conjugate
Brand: MM

suspension (Liquid), clone K121, Calbiochem^®

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关于此项目

NACRES:

NA.41

UNSPSC Code:

12352203

Clone:

K121, monoclonal

Species reactivity:

human, mouse, rat

Application:

—

Citations:

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属性

biological source

mouse

Quality Segment

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

K121, monoclonal

form

suspension (Liquid)

contains

≤0.1% sodium azide as preservative

species reactivity

human, mouse, rat

manufacturer/tradename

Calbiochem^®

storage condition

do not freeze

dilution

(Affinity Purification
Immunoprecipitation (15 µL beads/sample)
Immunoprecipitation (1-2 µg/mL)
Immunocytochemistry
Paraffin Sections)

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

说明

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Conjugated to agarose. Recognizes the exposed 5′ cap structure (2,2,7-trimethylguanosine) of small nuclear RNAs (snRNAs).

Recognizes the exposed 5′ cap structure (2,2,7-trimethylguanosine) of small nuclear RNAs (snRNAs). Also recognizes 7-methylguanosine-capped mRNA.

This Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Agarose Conjugate is validated for use in Affinity Purification, IP, IP, ICC, Paraffin Sections for the detection of 2,2,7-Trimethylguanosine.

Immunogen

2,2,7-trimethylguanosine

Application

Affinity Purification (see application references)

Immunoprecipitation (15 µl beads/sample)

Immunoprecipitation (1-2 µg/ml; see application references)

Immunocytochemistry (use Cat. No. NA02)

Paraffin Sections (use Cat. No. NA02)

Physical form

In PBS.

Analysis Note

Negative Control
Transfer RNA (tRNA)

Positive Control
Heterogenous nuclear RNA (hnRNA)

Other Notes

Also recognizes 7-methylguanosine-capped mRNA. 2,2,7-trimethylguanosine (Ab-1) is human, mouse, and rat reactive. This antibody reacts with the exposed 5′ cap structure of the snRNAs and the antibody can be used for the purification of snRNPs by immunoaffinity chromatography. All of the major snRNPs bind to the monoclonal antibody column and can be gently eluted by washing with 7-methyl guanosine. Antibody should be titrated for optimal results in individual sample types.

Casciola-Rosen, L.A., et al. 1994. J. Biol. Chem.269, 30757-30760.
Krainer, A.R. 1988. Nuc. Acids Res.16, 9415.
Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71.
Reddy, R. and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1.
Krainer, A.R. and Maniatis, T. 1985. Cell42, 725.
Kramer, A., et al. 1984. Cell38, 299.
Lerner, M.R., et al. 1980. Nature283, 220.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c