生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
K121, monoclonal
表单
suspension (Liquid)
包含
≤0.1% sodium azide as preservative
种属反应性
human, mouse, rat
制造商/商品名称
Calbiochem®
储存条件
do not freeze
dilution
(Affinity Purification
Immunoprecipitation (15 µL beads/sample)
Immunoprecipitation (1-2 µg/mL)
Immunocytochemistry
Paraffin Sections)
同位素/亚型
IgG1
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
一般描述
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Conjugated to agarose. Recognizes the exposed 5′ cap structure (2,2,7-trimethylguanosine) of small nuclear RNAs (snRNAs).
Recognizes the exposed 5′ cap structure (2,2,7-trimethylguanosine) of small nuclear RNAs (snRNAs). Also recognizes 7-methylguanosine-capped mRNA.
This Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Agarose Conjugate is validated for use in Affinity Purification, IP, IP, ICC, Paraffin Sections for the detection of 2,2,7-Trimethylguanosine.
免疫原
2,2,7-trimethylguanosine
应用
Affinity Purification (see application references)
Immunoprecipitation (15 µl beads/sample)
Immunoprecipitation (1-2 µg/ml; see application references)
Immunocytochemistry (use Cat. No. NA02)
Paraffin Sections (use Cat. No. NA02)
Immunoprecipitation (15 µl beads/sample)
Immunoprecipitation (1-2 µg/ml; see application references)
Immunocytochemistry (use Cat. No. NA02)
Paraffin Sections (use Cat. No. NA02)
外形
In PBS.
分析说明
Negative Control
Transfer RNA (tRNA)
Transfer RNA (tRNA)
Positive Control
Heterogenous nuclear RNA (hnRNA)
Heterogenous nuclear RNA (hnRNA)
其他说明
Also recognizes 7-methylguanosine-capped mRNA. 2,2,7-trimethylguanosine (Ab-1) is human, mouse, and rat reactive. This antibody reacts with the exposed 5′ cap structure of the snRNAs and the antibody can be used for the purification of snRNPs by immunoaffinity chromatography. All of the major snRNPs bind to the monoclonal antibody column and can be gently eluted by washing with 7-methyl guanosine. Antibody should be titrated for optimal results in individual sample types.
Casciola-Rosen, L.A., et al. 1994. J. Biol. Chem.269, 30757-30760.
Krainer, A.R. 1988. Nuc. Acids Res.16, 9415.
Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71.
Reddy, R. and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1.
Krainer, A.R. and Maniatis, T. 1985. Cell42, 725.
Kramer, A., et al. 1984. Cell38, 299.
Lerner, M.R., et al. 1980. Nature283, 220.
Krainer, A.R. 1988. Nuc. Acids Res.16, 9415.
Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71.
Reddy, R. and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1.
Krainer, A.R. and Maniatis, T. 1985. Cell42, 725.
Kramer, A., et al. 1984. Cell38, 299.
Lerner, M.R., et al. 1980. Nature283, 220.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Standard Handling (A)
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储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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