产品名称
Anti-p14ARF (Ab-2) Mouse mAb (14PO2), liquid, clone 14PO2, Calbiochem®
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
14PO2, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
should not react with
rat, mouse
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
Quality Level
Gene Information
human ... CDKN2A(1029)
Analysis Note
Negative Control
Normal sheep serum
Normal sheep serum
Positive Control
HeLa cells
HeLa cells
Application
Immnunoblotting (2-4 µg/ml)
Immunofluorescence (0.5 µg/ml)
Immunoprecipitation (2 µg/mg lysate)
Immunofluorescence (0.5 µg/ml)
Immunoprecipitation (2 µg/mg lysate)
Disclaimer
Toxicity: Standard Handling (A)
General description
Purified mouse monoclonal generated by immunizing BALB/c mice with the specified immunogen and fusing splonocytes with mouse myeloma cells. Recognizes the ~14 kDa p14ARF protein.
Recognizes the ~14 kDa p14ARF protein in HeLa cells. Sold under license of U.S. Patent 6,482,929.
This Anti-p14ARF (Ab-2) Mouse mAb (14PO2) is validated for use in Immnunoblotting, Immunofluorescence, Immunoprecipitation for the detection of p14ARF (Ab-2).
Immunogen
Human
recombinant, human p14ARF (p16β)
Legal Information
Sold under license of U.S. Patent 6,482,929.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Antibody should be titrated for optimal results in individual systems.
Bates, S., et al. 1998. Nature395, 124.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 10 mM PBS, 0.2% BSA, pH 7.4.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
L Miao et al.
Oncogene, 29(5), 711-722 (2009-11-11)
Although Myc-interacting zinc-finger protein-1 (Miz-1) is known to be a poxvirus and zinc-finger (POZ) transcription factor required for Myc transcriptional repression, additional regulatory function of Miz-1 is less well understood. Using a yeast two-hybrid screen, we identified human alternate reading
Stephen L Lessnick et al.
Cancer cell, 1(4), 393-401 (2002-06-28)
Ewing's sarcoma is associated with a fusion between the EWS and FLI1 genes, forming an EWS/FLI fusion protein. We developed a system for the identification of cooperative mutations in this tumor through expression of EWS/FLI in primary human fibroblasts. Gene
Wai Kei Kwok et al.
Carcinogenesis, 28(12), 2467-2475 (2007-08-11)
Recently, TWIST, a basic helix-loop-helix transcription factor, is suggested to be an oncogene because of its over-expression in many types of human cancer and its positive role in promoting cell survival. The aim of this study was to investigate the
Chongwei Xie et al.
FEBS letters, 586(4), 435-441 (2012-01-31)
ARF is the second most commonly inactivated tumor suppressor behind p53. It has been implicated in the control of cell proliferation, cell senescence, and tumor suppression. However, the detailed mechanism underlying the transcriptional control of ARF remains largely unknown. Here
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