biological source
mouse
Quality Level
antibody product type
primary antibodies
clone
NSF-1, monoclonal
form
liquid
contains
3 mM sodium azide as preservative
species reactivity
human, rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
General description
This product has been discontinued.
Recognizes the ~76 kDa NSF-1 protein in human fibroblasts, MDBK, and PC12 cells.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myeloma cells. Recognizes the ~76 kDa NSF-1 protein.
Immunogen
Human
recombinant, human N-ethylmaleimide-senstive factor
Application
ELISA (1:2000)
Immunoblotting (1:2000)
Immunoprecipitation (1:500)
Immunoblotting (1:2000)
Immunoprecipitation (1:500)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 150 mM NaCl, 20 mM sodium phosphate, 50% glycerol, pH 7.5.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
Human or rat fibroblasts, MDBK cells, or PC12 cells
Human or rat fibroblasts, MDBK cells, or PC12 cells
Other Notes
Sutton, R.B., et al. 1998. Nature395, 347.
Lemons, P.P., et al. 1997. Blood90, 1490.
Edelmann, L., et al. 1995. EMBO J.14, 224.
Südhof, T.C. 1995. Nature375, 645.
Whiteheart, S.W., et al. 1994. J. Cell Biol.126, 945.
Lemons, P.P., et al. 1997. Blood90, 1490.
Edelmann, L., et al. 1995. EMBO J.14, 224.
Südhof, T.C. 1995. Nature375, 645.
Whiteheart, S.W., et al. 1994. J. Cell Biol.126, 945.
This antibody is suitable for ELISA and immunoprecipitation under both native and denaturing conditions. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
10-13 - German Storage Class 10 to 13
法规信息
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