OP03
Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421)
liquid, clone PAb421, Calbiochem®
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关于此项目
NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
PAb421, monoclonal
Species reactivity:
rabbit, monkey, human, mouse, rat
Application:
Citations:
56
产品名称
Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421), liquid, clone PAb421, Calbiochem®
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
PAb421, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
rabbit, monkey, human, mouse, rat
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
dilution
(Frozen Sections (10 µg/mL,
Gel Shift
Immunoblotting (10 µg/mL,
Immunofluorescence (1-20 µg/mL,
Flow Cytometry (1-20 µg/mL)
Immunoprecipitation (1g per sample, )
isotype
IgG2a
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... TP53(7157)
Analysis Note
Positive Control
Breast carcinoma, A431 cells, or p53 standard (Cat. No. 506147)
Breast carcinoma, A431 cells, or p53 standard (Cat. No. 506147)
Negative Control
SK-OV-3 cells or normal skin
SK-OV-3 cells or normal skin
Application
Frozen Sections (10 µg/ml, see application references)
Gel Shift (see comments)
Immunoblotting (10 µg/ml, see application references)
Immunofluorescence (1-20 µg/ml, see application references)
Flow Cytometry (1-20 µg/ml)
Immunoprecipitation (1 μg per sample, see application references)
Gel Shift (see comments)
Immunoblotting (10 µg/ml, see application references)
Immunofluorescence (1-20 µg/ml, see application references)
Flow Cytometry (1-20 µg/ml)
Immunoprecipitation (1 μg per sample, see application references)
Disclaimer
Toxicity: Standard Handling (A)
General description
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells (see application references). Recognizes the ~53 kDa mutant and wild-type p53 proteins.
Recognizes the ~53 kDa mammalian wild-type and mutant p53 protein. Does not recognize phosphorylated or acetylated p53.
This Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) is validated for use in Frozen Sections, Gel Shift, Immunoblotting, IF, FC, IP for the detection of p53 (Ab-1) (Pantropic).
Immunogen
Epitope: within amino acids 376-378 of human p53
Mouse
partially purified mouse p53
Other Notes
El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Legros, Y., et al. 1994. Oncogene9, 2071.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA89, 7491.
Kastan, M.B., et al. 1992. Cell71, 587.
Lane, D.P. 1992. Nature358, 15.
Kastan, M.B., et al. 1991. Cancer Res.51 6304.
Crawford, L. and Harlow, E. 1982. J. Virol.41, 709.
Harlow, E., et al. 1981. J. Virol.39 861.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Legros, Y., et al. 1994. Oncogene9, 2071.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA89, 7491.
Kastan, M.B., et al. 1992. Cell71, 587.
Lane, D.P. 1992. Nature358, 15.
Kastan, M.B., et al. 1991. Cancer Res.51 6304.
Crawford, L. and Harlow, E. 1982. J. Virol.41, 709.
Harlow, E., et al. 1981. J. Virol.39 861.
Originally called clone L21. Does not recognize phosphorylated p53. For a gel shift assay, use Cat. No. OP03L and resuspend in 100 µl buffer. Wild-type p53 has a short half-life and is present in low amounts in cells. For immunoprecipitation, increasing the amount of sample to be immunoprecipitated and applied to the gel may help visualize wild-type p53, and short incubation times with 35S-Met (≤1 h) will help reduce background. For immunoblotting of wild-type p53, maximize sensitivity by concentrating samples by immunoprecipitation with Cat. No. OP03, then immunoblot using PC35 and chemiluminescent detection. This antibody recognizes the region encompassing Ser376 to Ser378 (see application references) and does not recognize phosphorylated p53 (see application references). Antibody should be titrated for optimal results in individual systems.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
M Schreiber et al.
Genes & development, 13(5), 607-619 (1999-03-11)
The c-jun proto-oncogene encodes a component of the mitogen-inducible immediate-early transcription factor AP-1 and has been implicated as a positive regulator of cell proliferation and G1-to-S-phase progression. Here we report that fibroblasts derived from c-jun-/- mouse fetuses exhibit a severe
C J Chiou et al.
Journal of virology, 67(10), 6201-6214 (1993-10-01)
The 80-kDa IE2 nuclear phosphoprotein encoded by the human cytomegalovirus (HCMV) major immediate-early (MIE) gene behaves both as a nonspecific transactivator of heterologous reporter genes and as a specific repressor of its own promoter-enhancer region. To begin to examine the
Sun-Young Choi et al.
International journal of oncology, 40(1), 277-286 (2011-09-03)
Proteins involved in the G1 phase of the cell cycle are aberrantly expressed, sometimes in mutated forms, in human cancers including human hepatocellular carcinoma.
Laura Fozzatti et al.
PloS one, 8(6), e67954-e67954 (2013-07-11)
Studies have suggested that the nuclear receptor corepressor 1 (NCOR1) could play an important role in human cancers. However, the detailed molecular mechanisms by which it functions in vivo to affect cancer progression are not clear. The present study elucidated
Irina M Bochkis et al.
PLoS genetics, 8(6), e1002770-e1002770 (2012-06-28)
Gene duplication is a powerful driver of evolution. Newly duplicated genes acquire new roles that are relevant to fitness, or they will be lost over time. A potential path to functional relevance is mutation of the coding sequence leading to
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