OP120
Anti-c-ErbB3 (Ab-5) Mouse mAb (H3.105.5)
liquid, clone H3.105.5, Calbiochem®
别名:
Anti-HER3, Anti-ErbB3, Anti-Erythroblastosis Virus
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关于此项目
UNSPSC Code:
12352203
Clone:
H3.105.5, monoclonal
Species reactivity:
human, mouse
Application:
—
Citations:
1
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
H3.105.5, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human, mouse
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
isotype
IgG1
shipped in
wet ice
Quality Level
Gene Information
human ... ERBB3(2065)
target post-translational modification
unmodified
General description
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myeloma NSO cells (see application references). Recognizes the ~185 kDa c-Erb B3/HER-3 protein.
Recognizes the c-ErbB3/HER 3 protein in T47D cells and breast and prostate carcinoma tissue.
This Anti-c-ErbB3 (Ab-5) Mouse mAb (H3.105.5) is validated for use in FC, Frozen Sections, Immunoblotting, IP, Neutralization Studies, Paraffin Sections for the detection of c-ErbB3 (Ab-5).
Immunogen
Human
recombinant, human ErbB3 extracellular domain
Application
Flow Cytometry (see comments)
Frozen Sections (not recommended)
Immunoblotting (not recommended)
Immunofluorescence (see comments)
Immunoprecipitation (10 µl/mg protein lysate)
Neutralization Studies (see application references)
Paraffin Sections (not recommended)
Frozen Sections (not recommended)
Immunoblotting (not recommended)
Immunofluorescence (see comments)
Immunoprecipitation (10 µl/mg protein lysate)
Neutralization Studies (see application references)
Paraffin Sections (not recommended)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 10 mM PBS, 0.2% BSA, pH 7.4.
Preparation Note
For long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
Analysis Note
Positive Control
T47D cells or breast or prostate carcinoma
T47D cells or breast or prostate carcinoma
Other Notes
Chen, X., et al. 1996. J. Biol. Chem.271, 7620.
Carraway, K.L., et al. 1994. J. Biol. Chem.269, 14303.
Ciardiello, F., et al. 1991. Proc. Natl. Acad. Sci. USA88, 7792.
Plowman, G.D., et al. 1990. Proc. Natl. Acad. Sci. USA87, 4905.
Kraus, J.H., et al. 1989. Proc. Natl. Acad. Sci. USA86, 9193.
Shoyab, M., et al. 1989. Science243, 1074.
Kawakami, T., et al. 1986. Mol. Cell. Biol.6, 4195.
Carraway, K.L., et al. 1994. J. Biol. Chem.269, 14303.
Ciardiello, F., et al. 1991. Proc. Natl. Acad. Sci. USA88, 7792.
Plowman, G.D., et al. 1990. Proc. Natl. Acad. Sci. USA87, 4905.
Kraus, J.H., et al. 1989. Proc. Natl. Acad. Sci. USA86, 9193.
Shoyab, M., et al. 1989. Science243, 1074.
Kawakami, T., et al. 1986. Mol. Cell. Biol.6, 4195.
Does not cross-react with c ErbB1 (EGFR), c-ErbB2/HER-2/neu or c-ErbB4/HER-4. c-ErbB3/HER-3 is overexpressed in a variety of tumors (e.g., breast, stomach, pancreas and colon). This antibody is particularly well suited for immunoprecipitating the unoccupied receptor. Reported to block neuregulin-1 β growth and signalling effects in ovarian cancer cell lines. This antibody has also been reported to work for flow cytometry and immunofluorescence. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Zsófia Pénzváltó et al.
Journal of mammary gland biology and neoplasia, 24(2), 149-162 (2019-02-28)
In order to develop a practical model of breast cancer, with in vitro and syngeneic, immune-intact, in vivo growth capacity, we established a primary cell line derived from a mammary carcinoma in the transgenic FVB/N-Tg(MMTV-ErbB2*)NDL2-5Mul mouse, referred to as "NDLUCD". The
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