生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
H3.105.5, monoclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
human, mouse
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
同位素/亚型
IgG1
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
基因信息
human ... ERBB3(2065)
一般描述
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myeloma NSO cells (see application references). Recognizes the ~185 kDa c-Erb B3/HER-3 protein.
Recognizes the c-ErbB3/HER 3 protein in T47D cells and breast and prostate carcinoma tissue.
This Anti-c-ErbB3 (Ab-5) Mouse mAb (H3.105.5) is validated for use in FC, Frozen Sections, Immunoblotting, IP, Neutralization Studies, Paraffin Sections for the detection of c-ErbB3 (Ab-5).
免疫原
Human
recombinant, human ErbB3 extracellular domain
应用
Flow Cytometry (see comments)
Frozen Sections (not recommended)
Immunoblotting (not recommended)
Immunofluorescence (see comments)
Immunoprecipitation (10 µl/mg protein lysate)
Neutralization Studies (see application references)
Paraffin Sections (not recommended)
Frozen Sections (not recommended)
Immunoblotting (not recommended)
Immunofluorescence (see comments)
Immunoprecipitation (10 µl/mg protein lysate)
Neutralization Studies (see application references)
Paraffin Sections (not recommended)
包装
Please refer to vial label for lot-specific concentration.
外形
In 10 mM PBS, 0.2% BSA, pH 7.4.
制备说明
For long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
分析说明
Positive Control
T47D cells or breast or prostate carcinoma
T47D cells or breast or prostate carcinoma
其他说明
Chen, X., et al. 1996. J. Biol. Chem.271, 7620.
Carraway, K.L., et al. 1994. J. Biol. Chem.269, 14303.
Ciardiello, F., et al. 1991. Proc. Natl. Acad. Sci. USA88, 7792.
Plowman, G.D., et al. 1990. Proc. Natl. Acad. Sci. USA87, 4905.
Kraus, J.H., et al. 1989. Proc. Natl. Acad. Sci. USA86, 9193.
Shoyab, M., et al. 1989. Science243, 1074.
Kawakami, T., et al. 1986. Mol. Cell. Biol.6, 4195.
Carraway, K.L., et al. 1994. J. Biol. Chem.269, 14303.
Ciardiello, F., et al. 1991. Proc. Natl. Acad. Sci. USA88, 7792.
Plowman, G.D., et al. 1990. Proc. Natl. Acad. Sci. USA87, 4905.
Kraus, J.H., et al. 1989. Proc. Natl. Acad. Sci. USA86, 9193.
Shoyab, M., et al. 1989. Science243, 1074.
Kawakami, T., et al. 1986. Mol. Cell. Biol.6, 4195.
Does not cross-react with c ErbB1 (EGFR), c-ErbB2/HER-2/neu or c-ErbB4/HER-4. c-ErbB3/HER-3 is overexpressed in a variety of tumors (e.g., breast, stomach, pancreas and colon). This antibody is particularly well suited for immunoprecipitating the unoccupied receptor. Reported to block neuregulin-1 β growth and signalling effects in ovarian cancer cell lines. This antibody has also been reported to work for flow cytometry and immunofluorescence. Antibody should be titrated for optimal results in individual systems.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Standard Handling (A)
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储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Zsófia Pénzváltó et al.
Journal of mammary gland biology and neoplasia, 24(2), 149-162 (2019-02-28)
In order to develop a practical model of breast cancer, with in vitro and syngeneic, immune-intact, in vivo growth capacity, we established a primary cell line derived from a mammary carcinoma in the transgenic FVB/N-Tg(MMTV-ErbB2*)NDL2-5Mul mouse, referred to as "NDLUCD". The
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