Immunoaffinity purified mouse monoclonal antibody, generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with X63AG8.653 mouse myeloma cells (see application references). Recognizes the ~53 kDa mutant and wild-type forms of p53.

This Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) Agarose Conjugate is validated for use in Affinity Chromatography, Immunoprecipitation for the detection of p53 (Ab-6) (Pantropic).

Affinity Chromatography (see comments)

Immunoprecipitation (15 µl beads/sample; see application references)

Please refer to vial label for lot-specific concentration.

Agarose suspension in PBS.

El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
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Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA89, 7491.
Lane, D.P. 1992. Nature358, 15.
Kastan, M.B., et al. 1991 Cancer Res.51, 6304.

Recognizes both mutant and wild-type p53 under denaturing and non-denaturing conditions. This antibody reacts weakly with rodent p53; we do not recommend it for rodent samples. Wild-type p53 has a short half life and is present in low amounts in cells. For immunoprecipitation increasing the amount of sample and labeling with ³⁵S-Met for less than or equal to 1 h will aid in visualizing wild-type p53. Agarose conjugate has also been reported to work with affinity chromotography. Antibody should be titrated for optimal results in individual systems.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)