Quality Level
biological source
mouse
antibody product type
primary antibodies
clone
DO-1, monoclonal
form
slurry (Liquid)
contains
≤0.1% sodium azide as preservative
species reactivity
feline, human
should not react with
mouse, rat
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
isotype
IgG2a
shipped in
wet ice
General description
Immunoaffinity purified mouse monoclonal antibody, generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with X63AG8.653 mouse myeloma cells (see application references). Recognizes the ~53 kDa mutant and wild-type forms of p53.
This Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) Agarose Conjugate is validated for use in Affinity Chromatography, Immunoprecipitation for the detection of p53 (Ab-6) (Pantropic).
Application
Affinity Chromatography (see comments)
Immunoprecipitation (15 µl beads/sample; see application references)
Immunoprecipitation (15 µl beads/sample; see application references)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
Agarose suspension in PBS.
Other Notes
El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Legros, Y., et al. 1994. Oncogene9, 2071.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kastan, M.B., et al. 1992. Cell71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA89, 7491.
Lane, D.P. 1992. Nature358, 15.
Kastan, M.B., et al. 1991 Cancer Res.51, 6304.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Legros, Y., et al. 1994. Oncogene9, 2071.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kastan, M.B., et al. 1992. Cell71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA89, 7491.
Lane, D.P. 1992. Nature358, 15.
Kastan, M.B., et al. 1991 Cancer Res.51, 6304.
Recognizes both mutant and wild-type p53 under denaturing and non-denaturing conditions. This antibody reacts weakly with rodent p53; we do not recommend it for rodent samples. Wild-type p53 has a short half life and is present in low amounts in cells. For immunoprecipitation increasing the amount of sample and labeling with 35S-Met for less than or equal to 1 h will aid in visualizing wild-type p53. Agarose conjugate has also been reported to work with affinity chromotography. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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