生物来源
mouse
质量水平
抗体产品类型
primary antibodies
克隆
DO-1, monoclonal
表单
slurry (Liquid)
包含
≤0.1% sodium azide as preservative
种属反应性
feline, human
请勿与下列物质发生反应
mouse, rat
制造商/商品名称
Calbiochem®
储存条件
do not freeze
同位素/亚型
IgG2a
运输
wet ice
储存温度
2-8°C
一般描述
Immunoaffinity purified mouse monoclonal antibody, generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with X63AG8.653 mouse myeloma cells (see application references). Recognizes the ~53 kDa mutant and wild-type forms of p53.
This Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) Agarose Conjugate is validated for use in Affinity Chromatography, Immunoprecipitation for the detection of p53 (Ab-6) (Pantropic).
应用
Affinity Chromatography (see comments)
Immunoprecipitation (15 µl beads/sample; see application references)
Immunoprecipitation (15 µl beads/sample; see application references)
包装
Please refer to vial label for lot-specific concentration.
外形
Agarose suspension in PBS.
其他说明
El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Legros, Y., et al. 1994. Oncogene9, 2071.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kastan, M.B., et al. 1992. Cell71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA89, 7491.
Lane, D.P. 1992. Nature358, 15.
Kastan, M.B., et al. 1991 Cancer Res.51, 6304.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Legros, Y., et al. 1994. Oncogene9, 2071.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kastan, M.B., et al. 1992. Cell71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA89, 7491.
Lane, D.P. 1992. Nature358, 15.
Kastan, M.B., et al. 1991 Cancer Res.51, 6304.
Recognizes both mutant and wild-type p53 under denaturing and non-denaturing conditions. This antibody reacts weakly with rodent p53; we do not recommend it for rodent samples. Wild-type p53 has a short half life and is present in low amounts in cells. For immunoprecipitation increasing the amount of sample and labeling with 35S-Met for less than or equal to 1 h will aid in visualizing wild-type p53. Agarose conjugate has also been reported to work with affinity chromotography. Antibody should be titrated for optimal results in individual systems.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Standard Handling (A)
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