生物来源
mouse
质量水平
抗体产品类型
primary antibodies
克隆
DF10, monoclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
human
请勿与下列物质发生反应
mouse, rat
制造商/商品名称
Calbiochem®
储存条件
do not freeze
同位素/亚型
IgG1
运输
wet ice
储存温度
2-8°C
一般描述
Purified mouse monoclonal antibody generated by immunizing F1 mice with the specified immunogen and fusing splenocytes with SP2/0 cells. Recognizes the ~21 kDa p21WAF1 protein.
Recognizes the ~21 kDa p21WAF1 protein in skin or colon tissue and in cells expressing wild-type p53 (e.g. Hs27 or U205 cells treated with DNA damaging agents).
免疫原
full-length, recombinant human p21WAF1
应用
Immunoblotting (1 µg/ml)
Immunoprecipitation (2 µg/sample)
Immunoprecipitation (2 µg/sample)
包装
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
分析说明
Positive Control
Any cell line expressing wild-type p53 (e.g. Hs27 or U2Os cells treated with DNA-damaging agents)
Any cell line expressing wild-type p53 (e.g. Hs27 or U2Os cells treated with DNA-damaging agents)
其他说明
Agarwal, M.L., et al. 1995 Proc. Natl. Acad. Sci. USA92, 8493.
Chen, Y.Q., et al. 1995. Int. J. Oncology7, 889.
Deng, C., et al. 1995 Cell82, 675.
El-Deiry, W.S., et al. 1995 Cancer Res.55, 2910.
Waldman, T., et al. 1995 Cancer Res.55, 5187.
El-Deiry, W.S., et al. 1994 Cancer Res.54, 1169.
Elbendary, A., et al. 1994 Cell Growth Diff.5, 1301.
Li, R., et al. 1994 Nature371, 534.
Michieli, P., et al. 1994 Cancer Res. 54, 3391.
Noda, A., et al.1994. Exp. Cell Res.211, 90.
El-Deiry, W.S., et al. 1993. Cell75 817.
Gu, Y., et al. 1993. Nature366, 707.
Harper, J.W., et al. 1993 Cell75, 805.
Xiong, Y., et al. 1993. Genes Devel.7, 1572.
Xiong, Y., et al. 1993. Nature366, 701.
Xiong, Y., et al. 1992. Cell71, 505.
Chen, Y.Q., et al. 1995. Int. J. Oncology7, 889.
Deng, C., et al. 1995 Cell82, 675.
El-Deiry, W.S., et al. 1995 Cancer Res.55, 2910.
Waldman, T., et al. 1995 Cancer Res.55, 5187.
El-Deiry, W.S., et al. 1994 Cancer Res.54, 1169.
Elbendary, A., et al. 1994 Cell Growth Diff.5, 1301.
Li, R., et al. 1994 Nature371, 534.
Michieli, P., et al. 1994 Cancer Res. 54, 3391.
Noda, A., et al.1994. Exp. Cell Res.211, 90.
El-Deiry, W.S., et al. 1993. Cell75 817.
Gu, Y., et al. 1993. Nature366, 707.
Harper, J.W., et al. 1993 Cell75, 805.
Xiong, Y., et al. 1993. Genes Devel.7, 1572.
Xiong, Y., et al. 1993. Nature366, 701.
Xiong, Y., et al. 1992. Cell71, 505.
May also detect unidentified higher molecular weight proteins by immunoblotting. Maximal p21WAF1 expression requires wild type p53 activity. Treatment of U2OS cells with DNA damaging agents induces wild type p53 expression which in turn activates WAF1 expression. Serum stimulation of quiescent cells will give low level WAF1 expression independent of p53 expression. This antibody can also immunoprecipitate proteins associated with p21WAF1. For immunoblotting applications, antigen/antibody complexes are best visualized using chemiluminescence. Antibody should be titrated for optimal results in individual systems.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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