biological source
mouse
antibody product type
primary antibodies
clone
DF10, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
should not react with
mouse, rat
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
isotype
IgG1
shipped in
wet ice
Quality Level
General description
Purified mouse monoclonal antibody generated by immunizing F1 mice with the specified immunogen and fusing splenocytes with SP2/0 cells. Recognizes the ~21 kDa p21WAF1 protein.
Recognizes the ~21 kDa p21WAF1 protein in skin or colon tissue and in cells expressing wild-type p53 (e.g. Hs27 or U205 cells treated with DNA damaging agents).
Immunogen
full-length, recombinant human p21WAF1
Application
Immunoblotting (1 µg/ml)
Immunoprecipitation (2 µg/sample)
Immunoprecipitation (2 µg/sample)
Packaging
Please refer to vial label for lot-specific concentration.
Analysis Note
Positive Control
Any cell line expressing wild-type p53 (e.g. Hs27 or U2Os cells treated with DNA-damaging agents)
Any cell line expressing wild-type p53 (e.g. Hs27 or U2Os cells treated with DNA-damaging agents)
Other Notes
Agarwal, M.L., et al. 1995 Proc. Natl. Acad. Sci. USA92, 8493.
Chen, Y.Q., et al. 1995. Int. J. Oncology7, 889.
Deng, C., et al. 1995 Cell82, 675.
El-Deiry, W.S., et al. 1995 Cancer Res.55, 2910.
Waldman, T., et al. 1995 Cancer Res.55, 5187.
El-Deiry, W.S., et al. 1994 Cancer Res.54, 1169.
Elbendary, A., et al. 1994 Cell Growth Diff.5, 1301.
Li, R., et al. 1994 Nature371, 534.
Michieli, P., et al. 1994 Cancer Res. 54, 3391.
Noda, A., et al.1994. Exp. Cell Res.211, 90.
El-Deiry, W.S., et al. 1993. Cell75 817.
Gu, Y., et al. 1993. Nature366, 707.
Harper, J.W., et al. 1993 Cell75, 805.
Xiong, Y., et al. 1993. Genes Devel.7, 1572.
Xiong, Y., et al. 1993. Nature366, 701.
Xiong, Y., et al. 1992. Cell71, 505.
Chen, Y.Q., et al. 1995. Int. J. Oncology7, 889.
Deng, C., et al. 1995 Cell82, 675.
El-Deiry, W.S., et al. 1995 Cancer Res.55, 2910.
Waldman, T., et al. 1995 Cancer Res.55, 5187.
El-Deiry, W.S., et al. 1994 Cancer Res.54, 1169.
Elbendary, A., et al. 1994 Cell Growth Diff.5, 1301.
Li, R., et al. 1994 Nature371, 534.
Michieli, P., et al. 1994 Cancer Res. 54, 3391.
Noda, A., et al.1994. Exp. Cell Res.211, 90.
El-Deiry, W.S., et al. 1993. Cell75 817.
Gu, Y., et al. 1993. Nature366, 707.
Harper, J.W., et al. 1993 Cell75, 805.
Xiong, Y., et al. 1993. Genes Devel.7, 1572.
Xiong, Y., et al. 1993. Nature366, 701.
Xiong, Y., et al. 1992. Cell71, 505.
May also detect unidentified higher molecular weight proteins by immunoblotting. Maximal p21WAF1 expression requires wild type p53 activity. Treatment of U2OS cells with DNA damaging agents induces wild type p53 expression which in turn activates WAF1 expression. Serum stimulation of quiescent cells will give low level WAF1 expression independent of p53 expression. This antibody can also immunoprecipitate proteins associated with p21WAF1. For immunoblotting applications, antigen/antibody complexes are best visualized using chemiluminescence. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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M L Agarwal et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(18), 8493-8497 (1995-08-29)
Increased expression of wild-type p53 in response to DNA damage arrests cells late in the G1 stage of the cell cycle by stimulating the synthesis of inhibitors of cyclin-dependent kinases, such as p21/WAF1. To study the effects of p53 without
Y Xiong et al.
Cell, 71(3), 505-514 (1992-10-30)
Human cyclin D1 has been associated with a wide variety of proliferative diseases but its biochemical role is unknown. In diploid fibroblasts we find that cyclin D1 is complexed with many other cellular proteins. Among them are protein kinase catalytic
R Li et al.
Nature, 371(6497), 534-537 (1994-10-06)
In mammalian cells, DNA damage increases the levels of the nuclear tumour-suppressor p53, resulting in elevated synthesis of p21, an inhibitor of cyclin-dependent kinases (CDK). p21 may also directly block DNA replication by inhibiting the proliferating-cell nuclear antigen (PCNA), an
Y Chen et al.
International journal of oncology, 7(4), 889-893 (1995-10-01)
The p21(WAF1) gene encodes a cyclin-dependent kinase inhibitor and plays an important role in controlling the cell cycle. Its expression can be induced through wild-type p53-dependent or -independent pathways. Since the p53-dependent pathway is disrupted in more than 50% of
Y Gu et al.
Nature, 366(6456), 707-710 (1993-12-16)
The major events of the cell division cycle are triggered by periodic changes in the activity of cyclin-dependent protein kinases (CDKs). In mammals, the members of the CDK family include CDK2 and CDC2, which are thought to be involved in
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