生物来源
rabbit
质量水平
抗体产品类型
primary antibodies
克隆
polyclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
human
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型
IgG
运输
wet ice
储存温度
−20°C
一般描述
Protein-A affinity purified rabbit polyclonal antibody. Recognizes the ~145 kDa c-Kit protein.
Recognizes the ~145 kDa c-Kit protein.
This Anti-c-Kit (961-976) Rabbit pAb is validated for use in Immunofluorescence, Paraffin Sections, Immunoblotting for the detection of c-Kit (961-976).
免疫原
a synthetic peptide (GSTASSSQPLLVHDDV) corresponding to amino acids 961-976 of human c-Kit
应用
Immunofluorescence (1-5 µg/ml)
Paraffin Sections (1-10 µg/ml, urea pre-treatment required, see comments and application references)
Immunoblotting (not recommended)
Paraffin Sections (1-10 µg/ml, urea pre-treatment required, see comments and application references)
Immunoblotting (not recommended)
包装
Please refer to vial label for lot-specific concentration.
分析说明
Negative Control
Human fibroblast cells or normal rabbit IgG
Human fibroblast cells or normal rabbit IgG
Positive Control
A172 or bone marrow cells
A172 or bone marrow cells
其他说明
For staining paraffin sections, pre-treat samples with 6 M urea at 95°C, 10 min. Antibody should be titrated for optimal results in individual sample types.
Lerner, N.B., et al. 1991. Blood77, 1876.
Tsai, M., et al. 1991. J. Exp. Med.174, 125.
Flanagan, J.G., and Leder, P., 1990. Cell63, 185.
Chabot, B., et al. 1988. Nature335, 88.
Geissler, E.N., et al. 1988. Cell55, 185.
Majumder, S., et al. 1988. Mol. Cell. Biol.8, 4896.
Qui, F., et al. 1988. EMBO J.7, 1003.
Yarden, Y., et al. 1987. EMBO J.6, 3341.
Besmer, P., et al. 1986. Nature320, 415.
Tsai, M., et al. 1991. J. Exp. Med.174, 125.
Flanagan, J.G., and Leder, P., 1990. Cell63, 185.
Chabot, B., et al. 1988. Nature335, 88.
Geissler, E.N., et al. 1988. Cell55, 185.
Majumder, S., et al. 1988. Mol. Cell. Biol.8, 4896.
Qui, F., et al. 1988. EMBO J.7, 1003.
Yarden, Y., et al. 1987. EMBO J.6, 3341.
Besmer, P., et al. 1986. Nature320, 415.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Standard Handling (A)
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