biological source
rabbit
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
Quality Level
General description
Recognizes the ~145 kDa c-Kit protein.
This Anti-c-Kit (961-976) Rabbit pAb is validated for use in Immunofluorescence, Paraffin Sections, Immunoblotting for the detection of c-Kit (961-976).
Protein-A affinity purified rabbit polyclonal antibody. Recognizes the ~145 kDa c-Kit protein.
Immunogen
a synthetic peptide (GSTASSSQPLLVHDDV) corresponding to amino acids 961-976 of human c-Kit
Application
Immunofluorescence (1-5 µg/ml)
Paraffin Sections (1-10 µg/ml, urea pre-treatment required, see comments and application references)
Immunoblotting (not recommended)
Paraffin Sections (1-10 µg/ml, urea pre-treatment required, see comments and application references)
Immunoblotting (not recommended)
Packaging
Please refer to vial label for lot-specific concentration.
Analysis Note
Negative Control
Human fibroblast cells or normal rabbit IgG
Human fibroblast cells or normal rabbit IgG
Positive Control
A172 or bone marrow cells
A172 or bone marrow cells
Other Notes
For staining paraffin sections, pre-treat samples with 6 M urea at 95°C, 10 min. Antibody should be titrated for optimal results in individual sample types.
Lerner, N.B., et al. 1991. Blood77, 1876.
Tsai, M., et al. 1991. J. Exp. Med.174, 125.
Flanagan, J.G., and Leder, P., 1990. Cell63, 185.
Chabot, B., et al. 1988. Nature335, 88.
Geissler, E.N., et al. 1988. Cell55, 185.
Majumder, S., et al. 1988. Mol. Cell. Biol.8, 4896.
Qui, F., et al. 1988. EMBO J.7, 1003.
Yarden, Y., et al. 1987. EMBO J.6, 3341.
Besmer, P., et al. 1986. Nature320, 415.
Tsai, M., et al. 1991. J. Exp. Med.174, 125.
Flanagan, J.G., and Leder, P., 1990. Cell63, 185.
Chabot, B., et al. 1988. Nature335, 88.
Geissler, E.N., et al. 1988. Cell55, 185.
Majumder, S., et al. 1988. Mol. Cell. Biol.8, 4896.
Qui, F., et al. 1988. EMBO J.7, 1003.
Yarden, Y., et al. 1987. EMBO J.6, 3341.
Besmer, P., et al. 1986. Nature320, 415.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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