biological source
rabbit
antibody product type
primary antibodies
clone
polyclonal
form
liquid
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
Quality Level
General description
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~140-145 Tie2 protein phosphorylated at Tyr1108 and Tyr1117.
This product has been discontinued.
Recognizes the ~140-145 Tie2 protein phosphorylated at Tyr1108 and Tyr1117 in colon carcinoma tissue.
Recognizes the ~140-145 Tie2 protein phosphorylated at Tyr1108 and Tyr1117 in colon carcinoma tissue.
Immunogen
a synthetic phosphopeptide [pYEKFTpYAGID(C)] corresponding to amino acids surrounding the Tyr1108/1117 phosphorylation sites 1108-1117 of human Tie2
Application
ELISA (see comments)
Paraffin Sections (1:2000-1:5000, no pre-treatment required)
Analysis Note
Positive Control
Colon carcinoma tissue
Colon carcinoma tissue
Other Notes
Hayes, A.J., et al. 1999. Microvasc. Res.58, 2224.
Jones, N., et al. 1999. J. Biol. Chem.274, 30896.
Valenzuela, D.M., et al. 1999. Proc. Natl. Acad. Sci. USA96, 1904.
Jones, N. and Dumont, D.J. 1998. Oncogene17, 1097.
Jones, N., et al. 1999. J. Biol. Chem.274, 30896.
Valenzuela, D.M., et al. 1999. Proc. Natl. Acad. Sci. USA96, 1904.
Jones, N. and Dumont, D.J. 1998. Oncogene17, 1097.
This antibody can be used in conjunction with a biotinylated phosphopeptide to measure the level of Tie2 activity by competition ELISA. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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