生物来源
rabbit
抗体产品类型
primary antibodies
克隆
polyclonal
表单
liquid
不包含
preservative
种属反应性
human
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型
IgG
运输
dry ice
储存温度
−20°C
一般描述
Anti-Adenine Nucleotide Translocase-1 (Ab-1) (2-11), rabbit polyclonal, recognizes ~35 kDa ANT-1 in doxorubicin-treated MCF-7 cells. It is validated for WB, immunofluorescence, and paraffin sections.
Purified rabbit polyclonal antibody. Recognizes the ~35 kDa ANT-1 protein.
Recognizes the ~35 kDa ANT-1 protein in doxorubicin-treated MCF-7 cells or breast carcinoma tissue.
- Antibody Target Gene Symbol: ANK3
- Target Synonym: 2900054D09RIK, AI314020, AnkG, Ankyrin-3, ANKYRIN-G, FLJ45464, MGC14049
- Entrez Gene Name: ankyrin 3, node of Ranvier (ankyrin G)
- Hu Entrez ID: 288 (Related Antibodies: NB20, AP1034, PC523, AP1057)
- Mu Entrez ID: 11735
- Rat Entrez ID: 361833
免疫原
Human
a synthetic peptide corresponding to amino acids 2-11 of human ANT-1
应用

Immunoblotting (2.5 g/ml, chemiluminescence)
Immunofluorescence (1 g/ml)
Paraffin Sections (1 g/ml, heat pre-treatment required)
包装
Please refer to vial label for lot-specific concentration.
外形
In 50 mM sodium phosphate buffer, 50% glycerol.
制备说明
Following initial thaw, aliquot and freeze (-20°C).
分析说明
Positive Control
doxorubicin-treated MCF7 cells, or breast carcinoma tissue
doxorubicin-treated MCF7 cells, or breast carcinoma tissue
其他说明
Bauer, M.K.A., et al. 1999. J. Cell Biol.147, 1493.
Marzo, I, et al. 1998. Science281, 2027.
Marzo, I, et al. 1998. J. Exp. Med.187, 1261.
Zoratti, M. and Szabo, I. 1995. Biochim. Biophys. Acta1231, 139.
Klingenberg, M. 1993. J. Bioenerg. Biomembr.25, 447.
Riccio, P., et al. 1975. FEBS Lett.56, 133.
Marzo, I, et al. 1998. Science281, 2027.
Marzo, I, et al. 1998. J. Exp. Med.187, 1261.
Zoratti, M. and Szabo, I. 1995. Biochim. Biophys. Acta1231, 139.
Klingenberg, M. 1993. J. Bioenerg. Biomembr.25, 447.
Riccio, P., et al. 1975. FEBS Lett.56, 133.
MCF7 cells treated with doxorubicin at 0.2 µg/ml for 24 h can be used as positive control for immunoblotting. Antibody should be titrated for optimal results in individual systems.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Standard Handling (A)
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