assay
≥97% (SDS-PAGE)
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
shipped in
wet ice
storage temp.
−20°C
Quality Level
General description
Recombinant, human heregulin expressed in E. coli. Human recombinant heregulin whose DNA sequence encoding the EGF domain of heregulin α (amino acids 177-241) expressed in and purified from E. coli. The expressed protein consists of 65 amino acids with a predicted molecular weight of approximately 7 kDa.
Application
Proliferation Studies
Biochem/physiol Actions
EC₅₀ ~20-40 ng/ml as determined by a cell proliferation assay using breast carcinoma cell line SK-BR-3 or in a serum-free cell proliferation assay using the human cell line MCF7.
Physical form
Lyophilized from PBS, 50 µg BSA/µg cytokine.
Preparation Note
Store lyophilized protein dessicated at -20°C. Lyophilized product should be reconstituted in sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin to prepare a stock solution of no less than 50 µg/ml. Following initial thaw, aliquot and freeze (-20°C).
Other Notes
It is recommended that the protein be titrated for optimal results in individual systems.
Riese, D.J., et al. 1996. J. Biol. Chem.271, 20047.
Holmes, W.E., et al. 1992. Science256, 1205.
Karey, K.P., et al. 1988. Cancer Res.48, 4083.
Holmes, W.E., et al. 1992. Science256, 1205.
Karey, K.P., et al. 1988. Cancer Res.48, 4083.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
存储类别
11 - Combustible Solids
wgk
WGK 3
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