PF125
BACE1, His•Tag®, Human, Recombinant, NSO Cells
Recombinant, human BACE-1 (amino acids 1-460) fused at the C-terminus to a His•Tag sequence and expressed in NSO cells. Supplied as a mixture of ~68-70 kDa proenzyme and ~65-67 kDa mature enzyme.
别名:
Human β-Secretase
登录 查看组织和合同定价。
选择尺寸
关于此项目
UNSPSC Code:
12352202
Form:
lyophilized
Assay:
≥90% (SDS-PAGE)
Recombinant:
expressed in NSO cells
recombinant
expressed in NSO cells
assay
≥90% (SDS-PAGE)
form
lyophilized
specific activity
≥1 pmol/min-μg protein
does not contain
preservative
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
≤1 EU/μg Endotoxin (EU/μg BACE-1)
solubility
water: 500 μg/mL
shipped in
wet ice
storage temp.
−20°C
Quality Level
General description
as measured by the cleavage of 10 μM MCA-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(DNP)-Arg-Arg-NH2 fluorescent substrate using 100 ng BACE-1 in 0.1 M sodium acetate (pH 4.0) at room temperature
Physical form
Lyophilized from sterile-filtered PBS.
Preparation Note
Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 4 months at -20°C or -70°C. Avoid freeze/thaw cycles of solutions.
Other Notes
Ermolieff, J. et al. 2000. Biochemistry39, 12450.
Lin, X. et al. 2000. Proc. Natl. Acad. Sci. USA97, 1456.
Sinha, S. et al. 1999. Nature402, 537.
Vassar, R. et al. 1999. Science286, 735.
Yan, R. et al. 1999. Nature402, 533.
Lin, X. et al. 2000. Proc. Natl. Acad. Sci. USA97, 1456.
Sinha, S. et al. 1999. Nature402, 537.
Vassar, R. et al. 1999. Science286, 735.
Yan, R. et al. 1999. Nature402, 533.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Amanda Gonzales et al.
Journal of neuroscience methods, 202(1), 70-76 (2011-09-06)
The aspartyl protease BACE1 is the rate limiting enzyme in the synthesis of amyloid beta, which accumulation in the human brain is a hallmark of Alzheimer's disease (AD). BACE1 has been proposed as a surrogate marker of AD; however, very
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持