PF125
BACE1, His•Tag®, Human, Recombinant, NSO Cells
Recombinant, human BACE-1 (amino acids 1-460) fused at the C-terminus to a His•Tag sequence and expressed in NSO cells. Supplied as a mixture of ~68-70 kDa proenzyme and ~65-67 kDa mature enzyme.
别名:
Human β-Secretase
重组
expressed in NSO cells
质量水平
方案
≥90% (SDS-PAGE)
表单
lyophilized
比活
≥1 pmol/min-μg protein
不包含
preservative
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
杂质
≤1 EU/μg Endotoxin (EU/μg BACE-1)
溶解性
water: 500 μg/mL
运输
wet ice
储存温度
−20°C
一般描述
as measured by the cleavage of 10 μM MCA-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(DNP)-Arg-Arg-NH2 fluorescent substrate using 100 ng BACE-1 in 0.1 M sodium acetate (pH 4.0) at room temperature
外形
Lyophilized from sterile-filtered PBS.
制备说明
Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 4 months at -20°C or -70°C. Avoid freeze/thaw cycles of solutions.
其他说明
Ermolieff, J. et al. 2000. Biochemistry39, 12450.
Lin, X. et al. 2000. Proc. Natl. Acad. Sci. USA97, 1456.
Sinha, S. et al. 1999. Nature402, 537.
Vassar, R. et al. 1999. Science286, 735.
Yan, R. et al. 1999. Nature402, 533.
Lin, X. et al. 2000. Proc. Natl. Acad. Sci. USA97, 1456.
Sinha, S. et al. 1999. Nature402, 537.
Vassar, R. et al. 1999. Science286, 735.
Yan, R. et al. 1999. Nature402, 533.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Standard Handling (A)
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Amanda Gonzales et al.
Journal of neuroscience methods, 202(1), 70-76 (2011-09-06)
The aspartyl protease BACE1 is the rate limiting enzyme in the synthesis of amyloid beta, which accumulation in the human brain is a hallmark of Alzheimer's disease (AD). BACE1 has been proposed as a surrogate marker of AD; however, very
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