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Merck
CN

PH03L

Anti-PTP, T Cell (Ab-1) Mouse mAb (CF4-1D)

lyophilized, clone CF4-1D, Calbiochem®

别名:

Anti-Protein Tyrosine Phosphatase, T cell

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UNSPSC Code:
12352203
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产品名称

Anti-PTP, T Cell (Ab-1) Mouse mAb (CF4-1D), lyophilized, clone CF4-1D, Calbiochem®

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

CF4-1D, monoclonal

form

lyophilized

does not contain

preservative

species reactivity

human

should not react with

mouse

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

isotype

IgG2a

shipped in

ambient

storage temp.

2-8°C

Quality Level

Analysis Note

Positive Control
CCRF-CEM cells

Application

Immunocytochemistry (see comments)

Immunoblotting (1-5 µg/ml)

Immunoprecipitation (1-2 µg/sample)

Disclaimer

Toxicity: Standard Handling (A)

General description

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells. Recognizes the PTPase protein.
Recognizes the T-cell PTPase protein.
This Anti-PTP, T Cell (Ab-1) Mouse mAb (CF4-1D) is validated for use in Immunocytochemistry, Immunoblotting, Immunoprecipitation for the detection of PTP, T Cell (Ab-1).

Immunogen

Human
recombinant, human T cell PTPase

Other Notes

Brown-Shimer, S., et al. 1992. Can. Res.52, 478.
Fischer, E.H., et al. 1991 Science253, 401.
Brown-Shimer, S., et al. Proc. Natl. Acad. Sci. USA87, 5148.
Chernoff, J., et al. 1990. Proc. Natl. Acad. Sci. USA87, 2735.
Cool, D.E., et al. 1990. Proc. Natl. Acad. Sci. USA87, 7280.
Charbonneau, H., et al. 1989. Proc. Natl. Acad. Sci. USA86, 5252.
Cool, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA86, 5257.
Charbonneau, H., et al. 1988. Proc. Natl. Acad. Sci. USA85, 7182.
This antibody has been reported to work for immunocytochemistry. Antibody should be titrated for optimal results in individual systems.

Physical form

Lyophilized from 20 mM sodium bicarbonate, 100 µg BSA.

Preparation Note

Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4 to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

10-13 - German Storage Class 10 to 13

法规信息

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Marianne R Spalinger et al.
The Journal of clinical investigation, 126(5), 1783-1800 (2016-04-05)
Inflammasomes form as the result of the intracellular presence of danger-associated molecular patterns and mediate the release of active IL-1β, which influences a variety of inflammatory responses. Excessive inflammasome activation results in severe inflammatory conditions, but physiological IL-1β secretion is
Michael M Swarbrick et al.
Endocrinology, 150(4), 1670-1679 (2009-01-24)
Protein tyrosine phosphatase (PTP)-1B antagonizes insulin signaling and is a potential therapeutic target for insulin resistance associated with obesity and type 2 diabetes. To date, studies of PTP-1B have been limited by the availability of specific antagonists; however, treatment of
Claudia Penafuerte et al.
Oncoimmunology, 6(6), e1321185-e1321185 (2017-07-07)
PTP1B and TC-PTP are highly related protein-tyrosine phosphatases (PTPs) that regulate the JAK/STAT signaling cascade essential for cytokine-receptor activation in immune cells. Here, we describe a novel immunotherapy approach whereby monocyte-derived dendritic cell (moDC) function is enhanced by modulating the
Hui Guo et al.
Acta pharmacologica Sinica, 39(3), 425-437 (2017-11-10)
STAT1 and STAT3 are two important members of the STAT (signal transducers and activators of transcription) protein family and play opposing roles in regulating cancer cell growth. Suppressing STAT3 and/or enhancing STAT1 signaling are considered to be attractive anticancer strategies.
Ronald R Marchelletta et al.
The Journal of clinical investigation, 131(17) (2021-10-09)
Genome-wide association studies revealed that loss-of-function mutations in protein tyrosine phosphatase non-receptor type 2 (PTPN2) increase the risk of developing chronic immune diseases, such as inflammatory bowel disease (IBD) and celiac disease. These conditions are associated with increased intestinal permeability

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