产品名称
Anti-PTP, T Cell (Ab-1) Mouse mAb (CF4-1D), lyophilized, clone CF4-1D, Calbiochem®
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
CF4-1D, monoclonal
form
lyophilized
does not contain
preservative
species reactivity
human
should not react with
mouse
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
isotype
IgG2a
shipped in
ambient
storage temp.
2-8°C
Quality Level
Analysis Note
Positive Control
CCRF-CEM cells
CCRF-CEM cells
Application
Immunocytochemistry (see comments)
Immunoblotting (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Immunoblotting (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Disclaimer
Toxicity: Standard Handling (A)
General description
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells. Recognizes the PTPase protein.
Recognizes the T-cell PTPase protein.
This Anti-PTP, T Cell (Ab-1) Mouse mAb (CF4-1D) is validated for use in Immunocytochemistry, Immunoblotting, Immunoprecipitation for the detection of PTP, T Cell (Ab-1).
Immunogen
Human
recombinant, human T cell PTPase
Other Notes
Brown-Shimer, S., et al. 1992. Can. Res.52, 478.
Fischer, E.H., et al. 1991 Science253, 401.
Brown-Shimer, S., et al. Proc. Natl. Acad. Sci. USA87, 5148.
Chernoff, J., et al. 1990. Proc. Natl. Acad. Sci. USA87, 2735.
Cool, D.E., et al. 1990. Proc. Natl. Acad. Sci. USA87, 7280.
Charbonneau, H., et al. 1989. Proc. Natl. Acad. Sci. USA86, 5252.
Cool, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA86, 5257.
Charbonneau, H., et al. 1988. Proc. Natl. Acad. Sci. USA85, 7182.
Fischer, E.H., et al. 1991 Science253, 401.
Brown-Shimer, S., et al. Proc. Natl. Acad. Sci. USA87, 5148.
Chernoff, J., et al. 1990. Proc. Natl. Acad. Sci. USA87, 2735.
Cool, D.E., et al. 1990. Proc. Natl. Acad. Sci. USA87, 7280.
Charbonneau, H., et al. 1989. Proc. Natl. Acad. Sci. USA86, 5252.
Cool, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA86, 5257.
Charbonneau, H., et al. 1988. Proc. Natl. Acad. Sci. USA85, 7182.
This antibody has been reported to work for immunocytochemistry. Antibody should be titrated for optimal results in individual systems.
Physical form
Lyophilized from 20 mM sodium bicarbonate, 100 µg BSA.
Preparation Note
Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4 to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10-13 - German Storage Class 10 to 13
法规信息
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