产品名称
PhosphoDetect Anti-PDGFRβ(pTyr⁷⁵¹) Rabbit pAb, liquid, Calbiochem®
biological source
rabbit
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
does not contain
preservative
species reactivity
rat, human, mouse
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pTyr751)
Quality Level
Gene Information
human ... PDGFRB(5159)
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC
Analysis Note
Positive Control
Breast carcinoma and PDGF-treated L6, HepG2, and NIH3T3 cells
Breast carcinoma and PDGF-treated L6, HepG2, and NIH3T3 cells
Application
Flow Cytometry (1:200)
Immunoblotting (1:1000)
Immunoprecipitation (1:50)
Paraffin Sections (1:50, heat pre-treatment required; see comments)
Disclaimer
Toxicity: Standard Handling (A)
General description
Rabbit polyclonal antibody purified by protein A and immunoaffinity chromatography. Recognizes the ~190 kDa PDGF receptor-β protein phosphorylated at Tyr751.
Recognizes the ~190 kDa PDGF receptor-β protein phosphorylated at Tyr751 in PDGF-stimulated L6, HepG2, and NIH3T3 cells.
This PhosphoDetect Anti-PDGFRβ(pTyr⁷⁵¹) Rabbit pAb is validated for use in FC, Immunoblotting, IP, Paraffin Sections for the detection of PDGFRβ(pTyr⁷⁵¹).
Immunogen
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr⁷⁵¹ phosphorylation site of human PDGF receptor-β, coupled to KLH
Other Notes
Betsholtz, C., et al. 2001. Bioessays23, 494.
Ostman, A. and Heldin, C.H. 2001. Adv. Cancer Res.80, 1.
Ramalingam, K., et al. 1995. Bioorg. Med. Chem.3, 1263.
Panyotou, G. et al. 1992. EMBO J.11, 4261.
Coughlin, S.R., et al. 1988. Prog. Clin. Biol. Res.266, 39.
Deuel, T.F., et al. 1988. Biofactors1, 213.
Ostman, A. and Heldin, C.H. 2001. Adv. Cancer Res.80, 1.
Ramalingam, K., et al. 1995. Bioorg. Med. Chem.3, 1263.
Panyotou, G. et al. 1992. EMBO J.11, 4261.
Coughlin, S.R., et al. 1988. Prog. Clin. Biol. Res.266, 39.
Deuel, T.F., et al. 1988. Biofactors1, 213.
For staining paraffin sections bring slides to a boil in 1 mM EDTA, pH 8.0 and continue to heat at sub-boiling temperature for 15 min; no cooling is necessary. Wash paraffin sections with 1X TBS, 0.1% Tween®-20 detergent. For immunoblotting membranes should be incubated with antibody diluted in 1X TBS, 5% BSA, 0.1% Tween-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.
Physical form
In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
Preparation Note
Do not aliquot.
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存储类别
10 - Combustible liquids
wgk
WGK 2
Nolan L Boyd et al.
Tissue engineering. Part A, 17(11-12), 1537-1548 (2011-02-03)
Microvascular mural or perivascular cells are required for the stabilization and maturation of the remodeling vasculature. However, much less is known about their biology and function compared to large vessel smooth muscle cells. We have developed lines of multipotent mesenchymal
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