PhosphoDetect Anti-PDGFRβ(pTyr⁷⁵¹) Rabbit pAb

Rabbit polyclonal antibody purified by protein A and immunoaffinity chromatography. Recognizes the ~190 kDa PDGF receptor-β protein phosphorylated at Tyr⁷⁵¹.

Recognizes the ~190 kDa PDGF receptor-β protein phosphorylated at Tyr⁷⁵¹ in PDGF-stimulated L6, HepG2, and NIH3T3 cells.

This PhosphoDetect Anti-PDGFRβ(pTyr⁷⁵¹) Rabbit pAb is validated for use in FC, Immunoblotting, IP, Paraffin Sections for the detection of PDGFRβ(pTyr⁷⁵¹).

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr⁷⁵¹ phosphorylation site of human PDGF receptor-β, coupled to KLH

Flow Cytometry (1:200)
Immunoblotting (1:1000)
Immunoprecipitation (1:50)
Paraffin Sections (1:50, heat pre-treatment required; see comments)

In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.

Do not aliquot.

Positive Control
Breast carcinoma and PDGF-treated L6, HepG2, and NIH3T3 cells

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Ramalingam, K., et al. 1995. Bioorg. Med. Chem.3, 1263.
Panyotou, G. et al. 1992. EMBO J.11, 4261.
Coughlin, S.R., et al. 1988. Prog. Clin. Biol. Res.266, 39.
Deuel, T.F., et al. 1988. Biofactors1, 213.

For staining paraffin sections bring slides to a boil in 1 mM EDTA, pH 8.0 and continue to heat at sub-boiling temperature for 15 min; no cooling is necessary. Wash paraffin sections with 1X TBS, 0.1% Tween^®-20 detergent. For immunoblotting membranes should be incubated with antibody diluted in 1X TBS, 5% BSA, 0.1% Tween-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.

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Toxicity: Standard Handling (A)