生物来源
rabbit
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
C25A9, monoclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
human
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
dilution
(Immunoblotting (1:1000)
Immunoprecipitation (1:50))
同位素/亚型
IgG
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
phosphorylation (pSer423/pSer425)
基因信息
human ... SMAD3(4088)
一般描述
Purified rabbit monoclonal antibody generated by immunizing rabbits with the specified immunogen and fusing splenocytes with an immortal cell line. Recognizes the ~52 kDa Smad3 protein phosphorylated at Ser423/425.
Recognizes the ~52 kDa Smad3 protein phosphorylated at Ser423/425 in HT-1080 cells treated with TGF-β1.
This PhosphoDetect Anti-Smad3 (pSer423/425) Rabbit mAb (C25A9) is validated for use in Immunoblotting, Immunoprecipitation for the detection of Smad3 (pSer423/425).
免疫原
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser 423/425 phosphorylation sites of Smad3, coupled to KLH.
应用
Immunoblotting (1:1000)
Immunoprecipitation (1:50)
外形
In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol pH 7.5.
其他说明
Attisano, L., et al. 2002. Science 296, 1646.
Moustakas, A., et al. 2001. J. Cell Sci. 114, 4359.
Wrana, J. 2000. Science 23, 1.
Moustakas, A., et al. 2001. J. Cell Sci. 114, 4359.
Wrana, J. 2000. Science 23, 1.
Does not cross-react with other Smad proteins. For immunoblotting incubate the membrane with antibody diluted with TBS containing 5% BSA, 0.1% Tween®-20 detergent overnight at 4°C with gentle shaking. Please note that this is a rabbit monoclonal antibody and requires the use of an anti-rabbit secondary antibody. The immunogen sequence is 100% conserved in mouse, rat, monkey, bovine, xenopus, and zebrafish Smad3, but cross-reactivity has not been tested. Antibody should be titrated for optimal results in individual systems.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC
免责声明
Toxicity: Standard Handling (A)
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
Amy M Gancarz et al.
Nature neuroscience, 18(7), 959-961 (2015-06-02)
Activin receptor signaling, including the transcription factor Smad3, was upregulated in the rat nucleus accumbens (NAc) shell following withdrawal from cocaine. Direct genetic and pharmacological manipulations of this pathway bidirectionally altered cocaine seeking while governing morphological plasticity in NAc neurons.
Beatriz Ballester et al.
International journal of molecular sciences, 22(12) (2021-07-03)
Several transmembrane mucins have demonstrated that they contribute intracellularly to induce fibrotic processes. The extracellular domain of MUC16 is considered as a biomarker for disease progression and death in IPF patients. However, there is no evidence regarding the signalling capabilities
Javier Milara et al.
Respiratory research, 23(1), 313-313 (2022-11-16)
Pulmonary hypertension (PH) associated to idiopathic pulmonary fibrosis (IPF) portends a poor prognosis. IL-11 has been implicated in fibrotic diseases, but their role on pulmonary vessels is unknown. Here we analyzed the contribution of IL-11 to PH in patients with
Javier Milara et al.
Mucosal immunology, 14(2), 377-388 (2020-09-06)
Several mucins are implicated in idiopathic pulmonary fibrosis (IPF); however, there is no evidence regarding the role of MUC4 in the development of IPF. Here we demonstrated that MUC4 was overexpressed in IPF patients (n = 22) compared with healthy subjects (n = 21)
Beatriz Ballester et al.
Oncotarget, 11(15), 1306-1320 (2020-04-29)
Pirfenidone is a pleiotropic molecule approved to treat idiopathic pulmonary fibrosis (IPF). Pirfenidone has demonstrated to downregulate transforming growth factor-β1 (TGF-β1) cellular effects. However, its anti-fibrotic mechanism remains unclear. Here, we aim to analyze the effects of pirfenidone on the
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