PhosphoDetect Anti-Smad3 (pSer^423/425) Rabbit mAb (C25A9)

Purified rabbit monoclonal antibody generated by immunizing rabbits with the specified immunogen and fusing splenocytes with an immortal cell line. Recognizes the ~52 kDa Smad3 protein phosphorylated at Ser^423/425.

Recognizes the ~52 kDa Smad3 protein phosphorylated at Ser^423/425 in HT-1080 cells treated with TGF-β1.

This PhosphoDetect Anti-Smad3 (pSer^423/425) Rabbit mAb (C25A9) is validated for use in Immunoblotting, Immunoprecipitation for the detection of Smad3 (pSer^423/425).

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser ^423/425 phosphorylation sites of Smad3, coupled to KLH.

Immunoblotting (1:1000)
Immunoprecipitation (1:50)

In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol pH 7.5.

Attisano, L., et al. 2002. Science 296, 1646.
Moustakas, A., et al. 2001. J. Cell Sci. 114, 4359.
Wrana, J. 2000. Science 23, 1.

Does not cross-react with other Smad proteins. For immunoblotting incubate the membrane with antibody diluted with TBS containing 5% BSA, 0.1% Tween^®-20 detergent overnight at 4°C with gentle shaking. Please note that this is a rabbit monoclonal antibody and requires the use of an anti-rabbit secondary antibody. The immunogen sequence is 100% conserved in mouse, rat, monkey, bovine, xenopus, and zebrafish Smad3, but cross-reactivity has not been tested. Antibody should be titrated for optimal results in individual systems.

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Toxicity: Standard Handling (A)