usage
sufficient for 100 tests
species reactivity (predicted by homology)
mammals
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
technique(s)
flow cytometry: suitable
input
sample type cultured cells
fluorescence
λex ~540 nm; λem ~570 nm
detection method
fluorometric
storage temp.
−20°C
Quality Level
General description
A convenient and sensitive method for the detection of activated caspase-3 in living cells. The fluorescent marker is DEVD-FMK conjugated to sulforhodamine. This cell-permeable, non-toxic inhibitor binds irreversibly to activated caspase-3 in apoptotic cells. The fluorescence intensity can be measured by fluorescence microscopy, fluorescence microplate reader, or flow cytometry.
The activation of caspases plays a central role in apoptosis. The Caspase-3 Detection Kit provides a convenient and sensitive means for detecting activated caspase-3 in situ in living cells. The assay utilizes a caspase-3 inhibitor (DEVD-FMK) conjugated to sulfo-rhodamine as the fluorescent in situ marker. Red-DEVD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-3 in apoptotic cells. The red fluorescence label allows for direct detection of activated caspase-3 in apoptotic cells by fluorescence microscopy, flow cytometry, or fluorescence plate reader.
Biochem/physiol Actions
A broad range of species
Features and Benefits
Assay Time: 1.5 h
Other Notes
Labeled Caspase-3 Inhibitor (Red-DEVD-FMK), Wash Buffer, Caspase-3 Inhibitor (Z-VAD-FMK), and a user protocol.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
存储类别
10 - Combustible liquids
法规信息
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