用途
sufficient for 100 tests
质量水平
种属反应性（根据同源性预测）
mammals
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
技术
flow cytometry: suitable
输入
sample type cultured cells
荧光
λex ~540 nm; λem ~570 nm
检测方法
fluorometric
储存温度
−20°C
一般描述
A convenient and sensitive method for the detection of activated caspase-3 in living cells. The fluorescent marker is DEVD-FMK conjugated to sulforhodamine. This cell-permeable, non-toxic inhibitor binds irreversibly to activated caspase-3 in apoptotic cells. The fluorescence intensity can be measured by fluorescence microscopy, fluorescence microplate reader, or flow cytometry.
The activation of caspases plays a central role in apoptosis. The Caspase-3 Detection Kit provides a convenient and sensitive means for detecting activated caspase-3 in situ in living cells. The assay utilizes a caspase-3 inhibitor (DEVD-FMK) conjugated to sulfo-rhodamine as the fluorescent in situ marker. Red-DEVD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-3 in apoptotic cells. The red fluorescence label allows for direct detection of activated caspase-3 in apoptotic cells by fluorescence microscopy, flow cytometry, or fluorescence plate reader.
生化/生理作用
A broad range of species
特点和优势
Assay Time: 1.5 h
其他说明
Labeled Caspase-3 Inhibitor (Red-DEVD-FMK), Wash Buffer, Caspase-3 Inhibitor (Z-VAD-FMK), and a user protocol.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
储存分类代码
10 - Combustible liquids
法规信息
新产品
此项目有
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持