S7803
CpG WIZ™ MGMT - 甲基化特异性PCR测定
The components of the CpG WIZ MGMT Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. Sufficient reagents are provided to analyze 25 samples.
别名:
MGMT甲基化检测, 甲基化PCR试剂盒
相关类别
一般描述
甲基化特异性PCR（MSP）是一种利用少量DNA灵敏检测异常基因甲基化的新技术（Herman，1996）。该过程采用最初的亚硫酸氢盐反应来修饰DNA，然后使用设计用于区分甲基化和未甲基化DNA的特异性引物进行PCR扩增。
应用
使用CpGenome DNA修饰试剂盒（目录号S7820）或CpGENOME 快速DNA修饰试剂盒（目录号S7824）可促进初始亚硫酸氢盐反应，而CpG WIZ MGMT扩增试剂盒包含所需的试剂用于基因特异性PCR扩增反应。
其他说明
U引物组5 μM 每个引物（25X）35 μL（中性帽）90513 -15°C至-25°C
M引物组5 μM 每个引物（25X）35 μL（红色帽）90514 -15°C至-25°C
W引物组5 μM 每个引物（25X）35 μL（绿色帽）90515 -15°C至-25°C
U对照DNA0.1 μg/μL 50 μL（白色帽）90393 -15°C至-25°C
M对照DNA0.1 μg/μL 50 μL（红色帽）90394 -15°C至-25°C
W对照DNA0.05 μg/μL 50 μL（绿色帽）90395 -15°C至-25°C
通用10X PCR缓冲液265 μL（蓝色帽）90396 -15°C至-25°C
法律信息
免责声明
储存分类代码
10 - Combustible liquids
法规信息
相关内容
"Epigenetics describes heritable changes in gene expression caused by non-genetic mechanisms instead of by alterations in DNA sequence. These changes can be cell- or tissue-specific, and can be passed on to multiple generations. Epigenetic regulation enriches DNAbased information, allowing a cell to vary its response across diverse biological and environmental contexts. Although epigenetic mechanisms are primarily centered in the nucleus, these mechanisms can be induced by environmental signals such as hormones, nutrients, stress, and cellular damage, pointing to the involvement of cytoplasmic and extracellular factors in epigenetic regulation."
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