biological source
Escherichia coli
conjugate
alkaline phosphatase
clone
monoclonal
mol wt
15.5 kDa
technique(s)
immunohistochemistry: suitable (Paraffin)
General description
for Savphire monomeric streptavidin with an N-terminal His-tag and a C-terminal FLAG® tag.
Immunogen
N/A
Application
SAvPhire Monomeric Streptavidin,Cat.#SAE0109, is a high-affinity recombinant variant of SA that minimizes the risk of aggregation, precipitation, and crosslinking in pre-incubation with biotinylated molecules and offers easier access to the desired targets, tested for use in IHC (Paraffin).
Biochem/physiol Actions
SAvPhire Monomeric Streptavidin expressed in E. coli and conjugated with alkaline phosphatase is suitable for immunodetection applications. SAvPhire Monomeric Streptavidin is expressed with an N-terminal His-tag and a C-terminal FLAG® tag.
Species Reactivity Note: Predicted to react with all species.
Species Reactivity Note: Predicted to react with all species.
Physical form
Monomeric Streptavidin in buffer containing 0.05 M Tris, 0.15 M NaCl, 1 mM MgCl2, 0.2% BSA, pH 8.0 with 0.1% sodium azide with 10% glycerol.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Immunohistochemistry (Paraffin) with Anti-NeuN antibody (ABN78) for detection of NeuN in mouse brain tissue sections.
Immunohistochemistry (Paraffin) Analysis (IHC(P)): A 1:500 dilution Anti-NeuN (ABN78) was used to stain sections of mouse cerebral cortex and signal was detected with Savphire monomeric streptavidin alkaline phosphatase conjugate (1:500 dilution) and Vulcan Fast Red.
Immunohistochemistry (Paraffin) Analysis (IHC(P)): A 1:500 dilution Anti-NeuN (ABN78) was used to stain sections of mouse cerebral cortex and signal was detected with Savphire monomeric streptavidin alkaline phosphatase conjugate (1:500 dilution) and Vulcan Fast Red.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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