color
green
material
polyethersulfone membrane, polypropylene housing
sterility
non-sterile
product line
Millex™, Samplicity®
feature
holdup volume≤ 80 μL (after air purge), hydrophilic
manufacturer/tradename
Millex™, Samplicity®
parameter
45 °C max. temp., 8.6 bar max. inlet pressure (125 psi)
technique(s)
HPLC: suitable, UV/Vis spectroscopy: suitable, analytical sample preparation: suitable, fast flow filtration: suitable, low protein-binding filtration: suitable
diam.
33 mm
filtration area
4.5 cm2
volume
1.7 mL
matrix
Millipore Express®
pore size
0.45 μm
input
sample type aqueous solution(s)
sample type mild organic solution(s)
bubble point
≥2 bar, air with water at 23 °C
Quality Level
fitting
female Luer-Lok® inlet connection, female Luer-Lok® inlet fitting, male Luer outlet slip
shipped in
ambient
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The Samplicity® filtration system was developed to filter up to 8 samples directly into standard HPLC vials using vacuum-based filtration. The samples can then be used in downstream HPLC analysis. Given the high sensitivity of high performance liquid chromatography (HPLC) and ultra-high pressure liquid chromatography (UHPLC) analyses, it is critical to ensure that the filtered sample is not contaminated with extractables. This application note reports the results of tests for extractables from Millex Samplicity® LCR (0.45 μm hydrophilic PTFE) and LG (0.20 μm hydrophilic PTFE) filters using three commonly used HPLC detectors. The detectors used were an ultraviolet/visible (UV-Vis) spectrophotometer, a mass spectrometer and a fluorescence detector.
"Liquid chromatography mass spectrometry (LC-MS) and gas chromatography mass spectrometry (GC-MS) are being increasingly used in analysis of samples, not only in the biotechnology and pharmaceutical industries, but also more generally in the analysis, identification and quantitation of complex samples containing low levels of analytes. The advantages of LC-MS or GC-MS over other analytical techniques include high sensitivityand specificity for analyte detection and quantitation."
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