Millex Samplicity^® Filter

The Samplicity® filtration system was developed to filter up to 8 samples directly into standard HPLC vials using vacuum-based filtration. The samples can then be used in downstream HPLC analysis. Given the high sensitivity of high performance liquid chromatography (HPLC) and ultra-high pressure liquid chromatography (UHPLC) analyses, it is critical to ensure that the filtered sample is not contaminated with extractables. This application note reports the results of tests for extractables from Millex Samplicity® LCR (0.45 μm hydrophilic PTFE) and LG (0.20 μm hydrophilic PTFE) filters using three commonly used HPLC detectors. The detectors used were an ultraviolet/visible (UV-Vis) spectrophotometer, a mass spectrometer and a fluorescence detector.

"Liquid chromatography mass spectrometry (LC-MS) and gas chromatography mass spectrometry (GC-MS) are being increasingly used in analysis of samples, not only in the biotechnology and pharmaceutical industries, but also more generally in the analysis, identification and quantitation of complex samples containing low levels of analytes. The advantages of LC-MS or GC-MS over other analytical techniques include high sensitivityand specificity for analyte detection and quantitation."