Huh-7.5 Tet-On Human Hepatocellular Carcinoma Cell Line

Huh-7 is a well differentiated hepatocyte-derived carcinoma cell line, isolated from the liver tumor of a 57-year-old Japanese male (Nakabayashi, 1982). Derived from Huh-7, the Huh-7.5 cell line is highly permissive for subgenomic and genomic Hepatitis C virus (HCV) RNA replication (Blight et al., 2002) and is now employed for high yield production of HCV and studies of antivirals on industrial scale. Huh-7.5 Tet-On Human Hepatocellular Carcinoma Cell Line was generated from Huh-7.5 cells using retroviral transduction to express the Tet-On 3G transactivator protein and enable subsequent doxycycline-regulated transgene expression to aid in the study of subcellular interactions between HCV and its host (Luna, 2015; Takacs, 2017).

Huh-7.5 Tet-On human hepatocellular carcinoma cell line is useful for Hepatitis C virus (HCV) studies and production.

Cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact licensing@milliporesigma.com.

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.