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Merck
CN

ST1521

Anti-ULK1 Mouse mAb (2H8)

liquid, clone 2H8, Calbiochem®

别名:

Anti-Unc-51-Like Kinase 1

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关于此项目

NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
2H8, monoclonal
Species reactivity:
human
Application:
Citations:
3
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shipped in

wet ice

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

2H8, monoclonal

form

liquid

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(ELISA (see coments)
Immunoblotting (1-5 µg/mL))

isotype

IgG1

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

Gene Information

human ... ULK1(8408)

General description

Purified mouse monoclonal antibody. Recognizes the ~125 kDa ULK1 protein.
Recognizes the ~125 kDa ULK1 protein in human colon tissue.
This Anti-ULK1 Mouse mAb (2H8) is validated for use in ELISA, Immunoblotting for the detection of ULK1.

Immunogen

A recombinant polypeptide corresponding to amino acids 602-716 of human ULK1, expressed as a GST fusion protein
NA

Application


ELISA (see coments)
Immunoblotting (1-5 g/ml)

Physical form

In PBS, pH 7.2.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Human colon tissue

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Regulatory Review (Z)

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存储类别

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Shunsaku Nakagawa et al.
European journal of pharmacology, 696(1-3), 143-154 (2012-10-02)
Inhibitors of mammalian target of rapamycin (mTOR) have immunosuppressive and anti-cancer effects, but their effects on the progression of kidney disease are not fully understood. Using cells from normal kidney epithelial cell lines, we found that the antiproliferative effects of
Jianjian Shi et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 33(6), 7348-7362 (2019-03-09)
In this study, we investigated the pathophysiological impact of Rho-associated coiled-coil-containing protein kinase (ROCK)1 and ROCK2 double deletion vs. single deletion on cardiac remodeling. Utilizing a cardiomyocyte-specific and tamoxifen-inducible MerCreMer recombinase (MCM), 3 mouse lines (MCM/ROCK1fl/fl/ROCK2fl/fl, MCM/ROCK1fl/fl, and MCM/ROCK2fl/fl) were
Sara Iachettini et al.
Cell death & disease, 9(10), 996-996 (2018-09-27)
Sirtuin 6 (SIRT6) is a member of the NAD+-dependent class III deacetylase sirtuin family, which plays a key role in cancer by controlling transcription, genome stability, telomere integrity, DNA repair, and autophagy. Here we analyzed the molecular and biological effects

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