biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
3C7, monoclonal
form
liquid
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(ELISA
Immunoblotting (1-5 µg/mL)
Immunocytochemistry (10 µg/mL)
Paraffin Sections (6 µg/mL))
isotype
IgG2a
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Purified mouse monoclonal antibody. Recognizes the ~90 kDa LEPRE1 protein.
Recognizes the ~90 kDa LEPRE1 protein in HeLa cells and human esophagus tissue.
This Anti-LEPRE1 Mouse mAb (3C7) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of LEPRE1.
Immunogen
A recombinant polypeptide corresponding to amino acids 1-100 of human LEPRE1, expressed as a GST fusion protein
Application



ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (6 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
HeLa cells, Human esophagus tissue
HeLa cells, Human esophagus tissue
Other Notes
Choi, JW, et al. 2009. PLoS Genet.5, e1000750.
Cabral, W,A., et al. 2007. Nat Genet.39, 359.
Cabral, W,A., et al. 2007. Nat Genet.39, 359.
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| ST1622-100UG | 04055977223699 |