生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
3C7, monoclonal
表单
liquid
不包含
preservative
种属反应性
human
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(ELISA
Immunoblotting (1-5 µg/mL)
Immunocytochemistry (10 µg/mL)
Paraffin Sections (6 µg/mL))
同位素/亚型
IgG2a
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
unmodified
一般描述
Purified mouse monoclonal antibody. Recognizes the ~90 kDa LEPRE1 protein.
Recognizes the ~90 kDa LEPRE1 protein in HeLa cells and human esophagus tissue.
This Anti-LEPRE1 Mouse mAb (3C7) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of LEPRE1.
免疫原
A recombinant polypeptide corresponding to amino acids 1-100 of human LEPRE1, expressed as a GST fusion protein
应用
ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (6 g/ml)
外形
In PBS, pH 7.2.
制备说明
Following initial thaw, aliquot and freeze (-20°C).
分析说明
Positive Control
HeLa cells, Human esophagus tissue
HeLa cells, Human esophagus tissue
其他说明
Choi, JW, et al. 2009. PLoS Genet.5, e1000750.
Cabral, W,A., et al. 2007. Nat Genet.39, 359.
Cabral, W,A., et al. 2007. Nat Genet.39, 359.
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Regulatory Review (Z)
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储存分类代码
12 - Non Combustible Liquids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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