CellASIC ONIX plate diploid yeast cells (4 chamber, 5-7 micron)

Microincubator for Long-Term, Live Cell Microscopy and Hypoxic Culture

Design dynamic experiments with the CellASIC™ ONIX Microfluidic Platform. With microfluidic precision, you can push the boundaries of your cell biology experiments in an in vivo-like environment. This new perfusion-based system enables you to program automated changes to culture conditions while maintaining optical access to cells through your microscope. You can even track individual cell responses over time by using a time-lapse-enabled microscope for truly dynamic cellular analysis.

We used the CellASIC™ ONIX MicroFluidic System, in conjunction with the M04S microfluidic plate, to achieve perfusion-based microenvironment control and study drug-induced cell death of 3D cultures of MCF7 cells in Matrigel®. The microfluidic chamber recreates the physiologic mass transport condition for optimized cell health. Four upstream fluidic channels allow controlled exposure of the cells to different solutions. The plate can also be cultured in a standard incubator using a dedicated gravity-driven flow channel. The cells are in contact with a #1.5 thickness (170 μm) optical glass surface, enabling high quality imaging using an inverted microscope. An integrated micro-incubator system delivers temperature and gas control to the microfluidic chambers.