Expand^™ 20 kb^PLUS PCR System, dNTPack

The Expand 20 kb^PLUS PCR System is composed of an enzyme mix containing thermostable Taq DNA polymerase and Tgo DNA polymerase, a thermostable DNA polymerase with proofreading activity. This powerful polymerase mixture and associated buffer system is designed to give a high yield of PCR product when fragments longer than 20 kb need to be amplified.
T/A cloning can also be performed.

The kit has control human genomic DNA and a set of control primers for amplifying a 23kb fragment. This test can be used to test template DNA and primer pair performance.
Use 3.75 U for a standard 50μL PCR.
Each dNTPack contains 10mM additive-free sodium salt nucleotides as a ready-to-use mix.
To obtain long PCR products, first evaluate length and purity of the DNA template. Handling all genomic DNA templates with care. Genomic DNA template should be larger than 50kb, as determined with agarose gel electrophoresis.

Choose Expand^™ 20 kb^PLUS PCR System when amplifying genomic DNA fragments up to 35 kb. Expand 20 kb^PLUS PCR System features a specifically optimized buffer and enzyme-blend mixture to amplify extra-long pieces of DNA (over 20 kb). Control primers and DNA produce fragments of 23 kb in length.

• PCR
• RT-PCR
• Large-fragment amplification

1 kit containing 7 components

Store supplied human control DNA at 2–8 °C, all other components at -15 to -25°C.

Each lot of Expand 20kb^PLUS PCR System is function-tested in PCR using human genomic DNA and specific primers for β-globin to amplify a 23kb fragment.

For life science research only. Not for use in diagnostic procedures.

Volume Activity: 5 U/μl

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Expand is a trademark of Roche