form
lyophilized
mol wt
330 Da
packaging
pkg of 5 U
manufacturer/tradename
Roche
absorbance ratio
A290/260 nm 0.03-0.12, A280/260 nm 0.28-0.39, A250/260 nm 0.84-0.94
storage temp.
−20°C (−15°C to −25°C)
General description
Poly(dA) is a polymer of polydeoxyadenylic acid and takes up a single stranded structure. It is susceptible to UV irradiation resulting in photoreaction due to the presence of adjacent deoxyadenylic acid units. Poly(dA) along with β-glucan is used for the conjugation and delivery of DNA into cell lines.
Application
Poly(dA) is suitable for use in:
- DNA polymerase assay and as a buffer component for the dilution of protein DNA complex for electrophoretic mobility shift assay (EMSA) studies
- as ssDNA substrate for DNA helicase gene 40 product (G40P) NTP hydrolysis
- in Pyrococcus abyssi DNA polymerase assay
Other Notes
1 A260 unit corresponds to 40 μg ssDNA.
Chain Length Mean chain length lot 20-17: 250-500 bases.
For life science research only. Not for use in diagnostic procedures.
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
A specific photoreaction in polydeoxyadenylic acid
Porschke D
Proceedings of the National Academy of Sciences of the USA, 70(9), 2683-2686 (1973)
Bacillus subtilis bacteriophage SPP1 hexameric DNA helicase, G 40 P, interacts with forked DNA
Ayora S, et al.
Nucleic Acids Research, 30(11), 2280-2289 (2002)
V Iyer et al.
The EMBO journal, 14(11), 2570-2579 (1995-06-01)
Many yeast promoters contain homopolymeric dA:dT sequences that affect nucleosome formation in vitro and are required for wild-type levels of transcription in vivo. Here, we show that poly(dA:dT) is a novel promoter element whose function depends on its intrinsic structure
The hyperthermophilic euryarchaeota Pyrococcus abyssi likely requires the two DNA polymerases D and B for DNA replication
Henneke G, et al.
Journal of molecular biology, 350(1), 53-64 (2005)
Human DNA polymerase lambda functionally and physically interacts with proliferating cell nuclear antigen in normal and translesion DNA synthesis
Maga G, et al.
The Journal of biological chemistry, 277(50), 48434-48440 (2002)
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