DNA Molecular Weight Marker V

Fragment mixture prepared by cleavage of pBR322 DNA with restriction endonuclease Hae III.

Size Range: 8 to 587 bp

DNA Molecular Weight Marker V is used for the size determination of DNA in agarose gels. It simplifies accurate molecular weight determination of double-stranded DNA fragments generated by:

<UL><LI>Restriction digests</LI>

<LI>PCR</LI>

<LI>RT-PCR</LI></UL>

Activator: Ammonia, pyridine, imidazole, all at pH >7; detergents

Working concentration: A suggested working ratio, for immediate use in coupling procedures, is 2 mg POD to 4 mg lgG (molar ratio = 2:1, POD:IgG).

Storage conditions (working solution): 2 to 8 °C
Once thawed we recommend further storage at 2 to 8 °C.

Store at -15–-25 °C. (until use)

50 μg = 1A₂₆₀ unit

For life science research only. Not for use in diagnostic procedures.

Ready-to-use solution, 250μg/ml, in TE buffer (10mM Tris-HCl, 1mM EDTA, pH 8.0)

The mixture contains 22 DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 8, 11, 18, 21, 51, 57, 64, 80, 89, 104, 123, 124, 184, 192, 213, 234, 267, 434, 458, 504, 540, and 587 as determined by computer analysis of the pBR322 sequence.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.