Name: PCR ELISA, DIG-Labeling
Brand: ROCHE

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About This Item

UNSPSC代码:

41116133

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表单

solution

用途

sufficient for 50 reactions

包装

kit of 1 (8 components)

制造商/商品名称

Roche

技术

nucleic acid labeling: suitable

运输

dry ice

储存温度

−20°C

一般描述

The PCR ELISA, DIG-Labeling kit is for nonradioactive labeling of DNA with digoxigenin (DIG)-deoxyuridine triphosphate (dUTP) by polymerase chain reaction (PCR). We recommend the use of this kit in combination with the PCR ELISA, DIG-Detection kit. This kit is part of a complete PCR ELISA system. The PCR ELISA system allows for convenient detection of PCR products in a microplate format. The PCR ELISA allows the sensitive and specific detection of PCR products in solution. It is approximately 100 times more sensitive than conventional ethidium bromide-stained agarose gels.

应用

PCR ELISA, DIG-Labeling is used for the detection and quantification of the PCR product. The convenient enzyme linked immunosorbent assay (ELISA) format makes this kit particularly suitable for determining the presence or absence of a specific target sequence in a large number of samples. By combining with other reagents and equipment, this system can be used for many molecular biology applications. For example:

In the presence of an appropriate target-specific, biotin-labeled capture probe, you can distinguish PCR products that differ by as little as a single base pair. Thus the PCR ELISA system (DIG-labeling + DIG-detection) can be used: to detect point mutations, deletions or insertions and to classify target sequences (e.g., as in HLA-typing or cell typing).
Preparation of suitable DIG-labeled standards and addition of a colorimetric detection system will allow you to quantify PCR products.
The microplate format used by the PCR ELISA system makes the system compatible with automated plate preparation and reading systems.
The microplate format combined with colorimetric detection allows quantification of the amount of template present in a sample when used in combination with suitable standards.
When using appropriate capture probes, the kit can be used for the typing of a target sequence (e.g., in HLA-typing or cell typing). The microplate format makes this kit particularly suited for automation.
The kit has been used in RT-PCR ELISA method to measure the dihydropyrimidine dehydrogenase (DPD) mRNA expression in liver and cell cycle phase distribution of bone marrow cells.

包装

1 kit containing 8 components.

质量

For best results run a negative control omitting template DNA to check for cross contamination from reagents.

其他说明

仅用于生命科学研究。不可用于诊断。

仅试剂盒组分

产品编号

说明

PCR DIG Labeling Mix, containing 2 mM dATP, dCTP, dGTP, 1.9 mM dTTP, and 0.1 mM Digoxigenin-dUTP

PCR Reaction Buffer, without MgCl_2, 100 mM Tris/HCl, 500 mM KCl, pH 8.3 (20 °C) 10x concentrated

MgCl₂ Stock Solution 25 mM

PCR Buffer, 100 mM Tris/HCl, 15 mM MgCl₂, 500 mM KCl, pH 8.3 (20 °C) 10x concentrated

Taq DNA Polymerase, in storage buffer: 20 mM Tris/HCl, 1 mM dithiothreitol, 0.1 mM EDTA, 0.5% Nonidet P40 (v/v), 50% Tween 20 (v/v), 0.5% glycerol (v/v), pH 8.0 (+4 °C) 5 U/μl