usage
sufficient for 96 tests
packaging
kit of 1
manufacturer/tradename
Roche
technique(s)
ELISA: suitable
storage temp.
2-8°C
General description
Erythropoietin (Epo) is a cytokine belonging to the hematopoietic cytokine superfamily. It has structural similarity to growth hormone and is encoded as a 193 amino acid polypeptide comprising of canonical leader sequence composed of 27 amino acid, and a carboxyl terminal arginine which is removed by posttranslational cleavage. Furthermore, Epo is produced in adult kidney and fetal liver and is involved in stimulating red blood cell production.
Immunogen
Recognized epitope not known.
Application
For research use only. Not for use in diagnostic procedures.
The EPO ELISA is designed for use in life science research studies as a method for the determination of EPO concentrations in plasma and serum; it is not intended for diagnostic procedures. The literature indicates that in certain forms of anemia, specifically in aplastic renal anemia, the regulation of EPO formation in the kidney appears to be intact. This may lead to an extremely high EPO concentration in serum of the respective individuals.
Exaggerated EPO levels, accompanied by a normal or even elevated hemoglobin concentration (and/or hematocrit values), have been found in a series of diseases (e.g., tumors of the kidney or liver, or in secondary forms of polycythemia). Polycythemia vera, however, is characterized by an abnormally increased red blood cell count in tandem with a low EPO serum level. The EPO ELISA is intended to help increase scientific knowledge about these relationships.
The EPO ELISA is designed for use in life science research studies as a method for the determination of EPO concentrations in plasma and serum; it is not intended for diagnostic procedures. The literature indicates that in certain forms of anemia, specifically in aplastic renal anemia, the regulation of EPO formation in the kidney appears to be intact. This may lead to an extremely high EPO concentration in serum of the respective individuals.
Exaggerated EPO levels, accompanied by a normal or even elevated hemoglobin concentration (and/or hematocrit values), have been found in a series of diseases (e.g., tumors of the kidney or liver, or in secondary forms of polycythemia). Polycythemia vera, however, is characterized by an abnormally increased red blood cell count in tandem with a low EPO serum level. The EPO ELISA is intended to help increase scientific knowledge about these relationships.
Biochem/physiol Actions
The ELISA system detects both natural and recombinant human EPO from CHO cells. No cross-reaction with other serum components has been found.
Packaging
1 kit containing 10 components.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Unit Conversion: The EPO concentration of unknown samples is calculated from a calibration curve. Each lot of standards (used for the calibration curve) is calibrated according to the 2. IRP WHO preparation (see lot-specific label). Conversion factor: 1 mlU/ml corresponds to 6.6 pg/ml. Conversion factor: 1 mU = 6.6 pg.
The normal range of a healthy human lies between 4 to 90 mIU/ml.
The normal range of a healthy human lies between 4 to 90 mIU/ml.
仅试剂盒组分
产品编号
说明
- hEPO Control Serum
- Anti-h-EPO-Peroxidase antibody
- 6 Recombinant h-EPO Standards 0 - approximately 200 mIU/ml
- Incubation Buffer ready-to-use
- Sample Buffer ready-to-use
- Washing Buffer
- TMB Substrate Solution ready-to-use
- TMB Stop Solution
- Microplate Modules, precoated with polyclonal anti-EPO antiserum from rabbit
- Self-adhesive Plate Cover Foils
查看所有结果 (10)
signalword
Warning
Hazard Classifications
Acute Tox. 4 - Aquatic Chronic 2 - Eye Irrit. 2 - Met. Corr. 1 Dermal - Skin Irrit. 2 - Skin Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
does not flashNot applicable
flash_point_c
does not flashNot applicable
法规信息
新产品
此项目有
Yuanyuan Zhang et al.
International journal of molecular sciences, 15(6), 10296-10333 (2014-06-12)
Erythropoietin (EPO) regulation of red blood cell production and its induction at reduced oxygen tension provides for the important erythropoietic response to ischemic stress. The cloning and production of recombinant human EPO has led to its clinical use in patients
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持