产品名称
逆转录酶扩增™, solution, recombinant, expressed in E. coli (AP401 (k)), sufficient for 20 reactions, sufficient for 100 reactions
recombinant
expressed in E. coli (AP401 (k))
form
solution
usage
sufficient for 100 reactions
sufficient for 20 reactions
packaging
pkg of 1,000 U (11785826001 [20 μl])
pkg of 5,000 U (11785834001 [100 μl])
manufacturer/tradename
Roche
parameter
42-43 °C optimum reaction temp.
technique(s)
RT-PCR: suitable
RT-qPCR: suitable
input
purified RNA
detection method
probe-based
storage temp.
−20°C
Application
Expand™逆转录酶已被用于实时PCR 分析，可以使分离的RNA转化，进入cDNA。它还被用于逆转录（RT）。Expand™逆转录酶也可用于分析 cDNA 的序列并与基因组序列进行比较。 可以获得有关间隔顺序（内含子）和基因组重组的重要信息。
Biochem/physiol Actions
热灭活：95 °C下处理2分钟
适用于3kb RT-PCR反应的逆转录酶
适用于3kb RT-PCR反应的逆转录酶
Features and Benefits
- 获得更多的全长cDNA转录本和更长的高达13.5kb的转录本
- 没有核糖核酸酶 H活性
- 高效地在二级结构中转录总RNA、mRNA、病毒RNA和富含RNA物质。
- 两步法 RT-PCR检测的理想选择
- 在PCR反应前不需要纯化cDNA
General description
Expand™逆转录酶是一种RNA指导的DNA聚合酶，经过优化在两步法 RT-PCR检测中可以将cDNA扩增为13.5 kb 片段。该酶是莫洛尼鼠白血病病毒逆转录酶（M-MuLV-RT）的基因工程产品。核糖核酸酶H序列中的A点突变会使核糖核酸酶H活性降低至无法检出的水平。与采用天然M-MuLV-RT相比可以得到明显的改进，包括全长cDNA转录本数量更多且转录本更长
Other Notes
一单位逆转录酶的定义为，在+37 °C下，以 poly(A)+ × (dT)15为底物，将10 nmol TMP整合为酸性不可溶产物所需的酶量。
体积活性：50 U/μl
体积活性：50 U/μl
仅用于生命科学研究。不可用于诊断。
Packaging
包含3个组分。
Legal Information
Expand is a trademark of Roche
仅试剂盒组分
产品编号
说明
- Expand Reverse Transcriptase 50 U/μl
- Expand Reverse Transcriptase Buffer 5x concentrated
- DTT Stock Solution 100 mM
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_c
does not flash
法规信息
常规特殊物品
此项目有
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M Hatzfeld et al.
The Journal of cell biology, 149(1), 209-222 (2000-04-04)
Plakophilin 1, a member of the armadillo multigene family, is a protein with dual localization in the nucleus and in desmosomes. To elucidate its role in desmosome assembly and regulation, we have analyzed its localization and binding partners in vivo.
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PloS one, 9(8), e105349-e105349 (2014-08-22)
The metabolic syndrome is a worldwide problem mainly caused by obesity. FTO was found to be a obesity-risk gene in humans and FTO deficiency in mice led to reduction in adipose tissue. Thus, FTO is an important factor for the
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