03997
聚（乙二醇）
tested according to Ph. Eur., 3,000
别名:
Macrogol 3,000, PEG
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关于此项目
线性分子式:
H(OCH2CH2)nOH
化学文摘社编号:
UNSPSC Code:
12161900
PubChem Substance ID:
MDL number:
InChI
1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2
InChI key
LYCAIKOWRPUZTN-UHFFFAOYSA-N
agency
tested according to Ph. Eur.
form
powder
mol wt
2700-3300
solubility
aliphatic hydrocarbons: slightly soluble, organic solvents: soluble
application(s)
pharmaceutical (small molecule)
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Application
Poly (ethylene glycol) was used for metabolic labeling and immunoprecipitation. It is suitable for radioimmunoassay.
Biochem/physiol Actions
Polyethylene glycol (PEG) also referred to as as Macrogol 3,000 or PEG is a condensation polymer of ethylene oxide and water. It enhances the refolding of extracted proteins. This enables characterization of crystallized proteins.
Preparation Note
Poly(ethylene glycol) dissolves in many organic solvents and is readily soluble in aromatic hydrocarbons. It also slightly dissolves in aliphatic hydrocarbons.
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
281.5 °F - closed cup
flash_point_c
138.6 °C - closed cup
ppe
Eyeshields, Gloves, type N95 (US)
F Galbiati et al.
The Journal of biological chemistry, 274(9), 5843-5850 (1999-02-20)
Here we investigate the molecular mechanisms that govern the targeting of G-protein alpha subunits to the plasma membrane. For this purpose, we used Gi1alpha as a model dually acylated G-protein. We fused full-length Gi1alpha or its extreme NH2-terminal domain (residues
A C Cuello et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 32(9), 947-957 (1984-09-01)
Monoclonal antibodies were produced following immunization of mice with either [Leu5]enkephalin-bovine serum albumin or [Met5]enkephalin-keyhold limpet hemocyanin conjugates. Two monoclonal antibodies coded NOC1 and NOC2, respectively, were derived. These monoclonal antibodies did not discriminate between Leu- and Met-enkephalin in either
J L Cleland et al.
The Journal of biological chemistry, 267(19), 13327-13334 (1992-07-05)
Polyethylene glycol (PEG) inhibited aggregation during refolding of bovine carbonic anhydrase B (CAB) through the formation of a nonassociating PEG-intermediate complex. Stoichiometric concentrations of PEG were required for complete recovery of active protein during refolding at aggregating conditions. For example
J L Cleland et al.
Bio/technology (Nature Publishing Company), 10(9), 1013-1019 (1992-09-01)
Previous studies on the refolding of recombinant bovine carbonic anhydrase B (CAB) indicated that polyethylene glycol (PEG) significantly enhanced the recovery of active protein by reducing aggregation. To further test the ability of PEG to enhance refolding, three recombinant human
Mark A Rice et al.
Acta biomaterialia, 5(1), 152-161 (2008-09-17)
Ultrasound has potential as a non-destructive analytical technique to provide real-time online assessments of matrix evolution in cell-hydrogel constructs used in tissue engineering. In these studies, chondrocytes were encapsulated in poly(ethylene glycol) hydrogels, and gel degradation was manipulated to provide
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