biological source
bovine
grade
Molecular Biology
product line
BioReagent
assay
≥90.0% (GE)
form
lyophilized powder
impurities
HIV I and HIVII, HCV and HBsAg, tested negative
solubility
H2O: slightly soluble
foreign activity
DNase, RNase, protease, and alkaline phosphatase, none detected
storage temp.
2-8°C
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Application
乙酰化的目的在于灭活 BSA 中通常存在的核酸酶。乙酰化 BSA 不能用作蛋白质标准品，因为酪氨酸残基乙酰化使得 Lowry 分析和其他类似蛋白质检测法无法显色。
Albumin from bovine serum has been used for electron and immunoelectron microscopy. It has also been used for isolating and culturing neonatal rat cardiac ventricular myocytes.
Biochem/physiol Actions
Bovine serum albumin also referred to as BSA or Fraction V is a protein isolated from cows. BSA can block vacant binding sites in enzyme-linked immunosorbent assay (ELISA) in both poly-l-lysine (PLL)-treated and non-treated microwells. It can also trigger insulin dependent diabetes mellitus in humans.
Preparation Note
为了灭活核酸酶，将 BSA 乙酰化来制备。
Albumin from bovine serum is slightly soluble in water.
Other Notes
可能实行销售限制
在酶免疫法制备溴尿苷共轭物的过程中，使用 5-溴-2-脱氧尿苷单克隆抗体来评估淋巴细胞增殖
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
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Enzyme immunoassay using a monoclonal antibody against 5-bromo-2-deoxyuridine for the assessment of lymphoid cell proliferation.
T Ternynck et al.
Methods in enzymology, 150, 117-129 (1987-01-01)
J D Marsh et al.
Circulation, 98(3), 256-261 (1998-08-11)
The role of androgens in producing cardiac hypertrophy by direct action on cardiac myocytes is uncertain. Accordingly, we tested the hypothesis that cardiac myocytes in adult men and women express an androgen receptor gene and that myocytes respond to androgens
Giovanna Andreola et al.
The Journal of experimental medicine, 195(10), 1303-1316 (2002-05-22)
The hypothesis that FasL expression by tumor cells may impair the in vivo efficacy of antitumor immune responses, through a mechanism known as 'Fas tumor counterattack,' has been recently questioned, becoming the object of an intense debate based on conflicting
J Karjalainen et al.
The New England journal of medicine, 327(5), 302-307 (1992-07-30)
Cow's milk has been implicated as a possible trigger of the autoimmune response that destroys pancreatic beta cells in genetically susceptible hosts, thus causing diabetes mellitus. Studies in animals have suggested that bovine serum albumin (BSA) is the milk protein
M Steinitz
Analytical biochemistry, 282(2), 232-238 (2000-06-30)
ELISA provides a highly sensitive procedure for quantitating antigens and antibodies. In that assay, microwells are coated initially with a specific ligand and then saturated with inert molecules to minimize nonspecific background. Coating can be improved by pretreating the microwells
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