06212
七叶苷水合物 水合物
analytical standard
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关于此项目
经验公式（希尔记法）:
C15H16O9 · xH2O
化学文摘社编号:
分子量:
340.28 (anhydrous basis)
NACRES:
NA.24
PubChem Substance ID:
UNSPSC Code:
85151701
EC Number:
207-646-4
MDL number:
InChI key
CQYPGSKIFJFVDQ-QWFKVUSTSA-N
InChI
1S/C15H16O9.H2O/c16-5-10-12(19)13(20)14(21)15(24-10)23-9-3-6-1-2-11(18)22-8(6)4-7(9)17;/h1-4,10,12-17,19-21H,5H2;1H2/t10-,12-,13+,14-,15-;/m1./s1
grade
analytical standard
assay
≥98.0% (HPLC)
optical activity
[α]/D -40.5±2.5°, c = 5% in pyridine
shelf life
limited shelf life, expiry date on the label
impurities
≤11% water
application(s)
food and beverages
format
neat
Quality Level
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Application
Esculin hydrate may be used as an analytical standard for:
- The detection of glucosidase inhibitors by thin-layer chromatography (TLC) bioautographic method.
- Library screening in the characterization of novel cellulase genes from uncultured microorganisms.
- Evaluation of mutagenic and antimutagenic activities of oligorutin and oligoesculin.
- Detecting β-glucosidase activity in the identification of novel cellulase genes from uncultured microorganisms in rabbit cecum.
存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Yi Feng et al.
Applied microbiology and biotechnology, 75(2), 319-328 (2007-01-12)
A metagenomic cosmid library was prepared in Escherichia coli from DNA extracted from the contents of rabbit cecum and screened for cellulase activities. Eleven independent clones expressing cellulase activities (four endo-beta-1,4-glucanases and seven beta-glucosidases) were isolated. Subcloning and sequencing analysis
Mario O Salazar et al.
Phytochemical analysis : PCA, 18(3), 209-212 (2007-05-16)
A new bioautographic assay suitable for the localisation of beta-glucosidase inhibitors present in a complex matrix is described. Enzyme activity was detected using esculin as the substrate to produce esculetin, which reacts with ferric ion to form a brown complex.
Fengchao Wang et al.
Microbiological research, 164(6), 650-657 (2009-02-24)
Four different environmental DNA libraries were prepared from microbial consortium collected from forest soil, dung of elephant, cow rumen and rotted tree. Seven independent clones specifying cellulase activities (five endo-beta-1,4-glucanases and two beta-glucosidases) were isolated and identified. Sequence analysis of
Ghada Ben Rhouma et al.
Food chemistry, 135(3), 1700-1707 (2012-09-08)
Rutin and esculin have been polymerised by laccase. Five fractions with M(w)¯ between 2127.42 and 8331.85g/mol for oligorutins, and between 688.12 and 6973g/mol for oligoesculins, were obtained. Fourier transformed infrared analysis showed that oligorutins were formed through C-C, C-O and
I Diezhandino et al.
Food chemistry, 168, 134-141 (2014-08-31)
The aim of this work was to study the microbiological, physico-chemical and proteolytic changes in Valdeón blue-veined cheese during ripening. Eight replicas of cheese were produced and a total of 48 cheeses were analysed. Lactic acid bacteria, mainly lactococci, were
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