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Merck
CN

07980

5-Aminofluorescein

p.a., ≥90% (HPLC)

别名:

Fluoresceinamine isomer I

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经验公式(希尔记法):
C20H13NO5
化学文摘社编号:
分子量:
347.32
UNSPSC Code:
12171500
EC Number:
222-043-6
Beilstein/REAXYS Number:
48395
MDL number:
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grade

p.a.

assay

≥90% (HPLC)

impurities

≤1% 6-isomer (HPLC)

suitability

suitable for fluorescence

SMILES string

Nc1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35

Application

The two isomers are produced by the usual synthesis. Most publications imply they are interchangeable when used for the preparation of the isothiocyanate for fluorescent antibody work. In fact, most commercial preparations are not even labeled as to whether or not they are mixtures.

Other Notes

All known glycosaminoglycuronans react with this fluorescein derivative to yield fluorescent derivatives
Note: Do not confuse with fluorescamine.

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A Ogamo et al.
Carbohydrate research, 105(1), 69-85 (1982-07-01)
The uronic acid residues of all known glycosaminoglycuronans reacted with 5-aminofluorescein to yield fluorescent glycosaminoglycuronan derivatives, which showed fluorescence characteristics identical to those of fluorescein or 5-acetamidofluorescein. The fluorescent products could be purified by chromatography on Octyl-Sepharose; three preparations of

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