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Merck
CN

09688

乙酸铵

BioUltra, ≥99.0% (calc. based on dry substance, T)

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关于此项目

线性分子式:
CH3CO2NH4
化学文摘社编号:
分子量:
77.08
UNSPSC Code:
12352200
PubChem Substance ID:
eCl@ss:
39021908
EC Number:
211-162-9
Beilstein/REAXYS Number:
4186741
MDL number:
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grade

Molecular Biology

product line

BioUltra

assay

≥99.0% (calc. based on dry substance, T)

form

solid

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤2% water

ign. residue

≤0.01% (as SO4)

pH

6.5-7.5 (25 °C, 1 M in H2O)

mp

110-112 °C (dec.) (lit.)

solubility

H2O: 1 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤5 mg/kg, nitrate (NO3-): ≤10 mg/kg, sulfate (SO42-): ≤10 mg/kg

cation traces

Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤2 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

λ

1 M in H2O

SMILES string

N.CC(O)=O

UV absorption

λ: 260 nm Amax: ≤0.015, λ: 280 nm Amax: ≤0.010

InChI

1S/C2H4O2.H3N/c1-2(3)4;/h1H3,(H,3,4);1H3

InChI key

USFZMSVCRYTOJT-UHFFFAOYSA-N

Application

Ammonium acetate can be used to study molecular biology, reagents, protein structural analysis, proteomics and x-ray crystallography. Ammonium acetate has been used in a study to develop an easy, rapid, low-cost and effective cetyltrimethylammonium bromide (CTAB)-ammonium acetate method for high-quality RNA isolation from polysaccharide- and polyphenol-rich cotton tissues. Ammonium acetate has also been used in a study to develop and validate a simple and rapid high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) method for the determination of 4-p-anisamidobutyric acid (ABA; or N-anysoyl-γ-aminobutiryc acid, N-anisoyl-GABA), a major active metabolite of aniracetam, in human plasma.

Other Notes

Alternative to sodium acetate in ethanol precipitation of nucleic acids. Prevents precipitation of deoxyribonucleoside triphosphates.; Useful for removal of untreated triphosphates from the products of reverse transcriptase, DNA polymerase or terminal polymerase-catalyzed reactions.


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存储类别

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

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分析证书(COA)

Lot/Batch Number

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Precipitation of nucleic acids.
D M Wallace
Methods in enzymology, 152, 41-48 (1987-01-01)
Jessica Z Bereszczak et al.
Protein science : a publication of the Protein Society, 23(7), 884-896 (2014-04-10)
Hepatitis B virus core-antigen (capsid protein) and e-antigen (an immune regulator) have almost complete sequence identity, yet the dimeric proteins (termed Cp149d and Cp(-10)149d , respectively) adopt quite distinct quaternary structures. Here we use hydrogen deuterium exchange-mass spectrometry (HDX-MS) to
Elena N Kitova et al.
Journal of the American Society for Mass Spectrometry, 25(11), 1908-1916 (2014-08-20)
A semiquantitative electrospray ionization mass spectrometry (ESI-MS) binding assay suitable for analyzing mixtures of oligosaccharides, at unknown concentrations, for interactions with target proteins is described. The assay relies on the differences in the ratio of the relative abundances of the



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货号GTIN
234117-25G04061838784322