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Merck
CN

21022

三氟乙酸铯

BioUltra, ≥99.0% (NT)

别名:

CsTFA, 三氟乙酸 铯盐

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线性分子式:
CF3COOCs
化学文摘社编号:
分子量:
245.92
UNSPSC Code:
12352200
Beilstein/REAXYS Number:
4161420
MDL number:
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InChI key

RJYSYRSELCQCSO-UHFFFAOYSA-M

SMILES string

[Cs+].[O-]C(=O)C(F)(F)F

InChI

1S/C2HF3O2.Cs/c3-2(4,5)1(6)7;/h(H,6,7);/q;+1/p-1

grade

Molecular Biology

product line

BioUltra

assay

≥99.0% (NT)

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

pH

9.0-10.5 (25 °C, 2 M in H2O)

mp

114-116 °C (lit.)

solubility

H2O: 2 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤500 mg/kg, sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤10 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

λ

2 M in H2O

UV absorption

λ: 260 nm Amax: 0.09, λ: 280 nm Amax: 0.04

Other Notes

Medium in density gradient centrifugation for the separation of DNA, RNA and protein, and for plasmid isolation; Isolation of mRNA prior to the construction of full-length cDNA libraries

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P E Mirkes
Analytical biochemistry, 148(2), 376-383 (1985-08-01)
A procedure for the simultaneous banding of cellular DNA, RNA, and protein by centrifugation in cesium trifluoroacetate (CsTFA) gradients is described. Starting with homogenates of Day 11 rat embryos, this procedure was used to separate total DNA, RNA, and protein.
D S Zarlenga et al.
Analytical biochemistry, 162(2), 569-574 (1987-05-01)
A rapid method for simultaneously banding preparative amounts of RNA and DNA from Trichinella spiralis muscle larvae by isopycnic centrifugation in cesium trifluoroacetate (CsTFA) is described. Larvae were homogenized in guanidinium isothiocyanate and the DNA, RNA, glycogen, and denatured protein
K Andersson et al.
Plasmid, 13(1), 78-80 (1985-01-01)
Plasmids extracted from bacterial cells by alkaline extraction can easily be isolated from linear DNA by isopycnic centrifugation in CsTFA. This is a fast and simple method which circumvents the use of the intercalating dye, ethidium bromide, and consequently the
High-efficiency cloning of full-length cDNA; construction and screening of cDNA expression libraries for mammalian cells.
H Okayama et al.
Methods in enzymology, 154, 3-28 (1987-01-01)

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