25328
4-氯-1-萘酚
≥99.0%
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线性分子式:
ClC10H6OH
化学文摘社编号:
分子量:
178.61
UNSPSC Code:
12171500
NACRES:
MA.02
PubChem Substance ID:
EC Number:
210-068-5
Beilstein/REAXYS Number:
509750
MDL number:
description
for analytical purposes
Quality Level
assay
≥99.0% (HPLC), ≥99.0%
mp
118-120 °C, 118-121 °C (lit.)
solubility
methanol: 50 mg/mL, clear, colorless to very faintly yellow
fluorescence
λex 310 nm; λem 375 nm in ethanol
SMILES string
Oc1ccc(Cl)c2ccccc12
InChI
1S/C10H7ClO/c11-9-5-6-10(12)8-4-2-1-3-7(8)9/h1-6,12H
InChI key
LVSPDZAGCBEQAV-UHFFFAOYSA-N
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General description
4-Chloro-1-naphthol is primarily used for the detection of proteins, it gives a characteristic purple precipitate and the reactions are easily controllable. It can be used as a substitute for benzidine compounds, which are considered to be carcinogenic.
Application
4-chloro-1-naphthol along with N,N′-dimethyl-p-phenylendiamine may be used in an oxidative coupling reaction for visualization of antigen-antibody complexes on the nitrocellulose membrane. 4-Chloro-1-naphthol may also be used in scanning electrochemical microscopy (SECM), for imaging of DNA hybridization on microscopic polypyrrole patterns. It was used in immunoblotting analysis during purification of native specific proteins of Taenia crassiceps cysticerci antigens obtained by immunoaffinity chromatography.
electrophoresis test: suitable for peroxidase detection on blotting membranes
Other Notes
可用于过氧化物酶染色实验。在等电点聚焦和免疫印迹实验中转入硝化纤维膜后检测特定抗原；在斑点免疫结合试验中用于检测和定量人 IgE2；可用于快速多色蛋白质印迹实验
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
法规信息
新产品
此项目有
Noeli Maria Espíndola et al.
Journal of clinical microbiology, 43(7), 3178-3184 (2005-07-08)
Monoclonal antibodies (MAb) against Taenia crassiceps and Taenia solium cysticerci were produced and showed cross-reactivity with a 14-kDa protein from T. solium and with 18- and 14-kDa proteins from T. crassiceps. These MAbs and antibodies from cerebrospinal fluid (CSF) as
L. Miribel et al.
Protides Biol. Fluid Proc. Colloq., 34, 753-753 (1986)
N Lee et al.
Journal of immunological methods, 106(1), 27-30 (1988-01-21)
A multicolor Western blotting technique was developed, by which different kinds or different subtypes of interferon were identified with different colors on a single Western blot. This was achieved by sequentially applying different sets of probing antibodies, enzyme-conjugated developing antibodies
Endocrine Methods.
Meurant G.
Science, 214-215 (1996)
C S Hong et al.
Journal of immunological methods, 95(2), 195-202 (1986-12-24)
This paper describes a dot immunobinding assay for determining total human IgE with a tandem of monoclonal anti-IgE antibodies. Minute quantities of monoclonal anti-IgE antibodies were adsorbed on nitrocellulose discs. IgE bound to this solid phase monoclonal anti-IgE antibody was
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