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25668

氯仿 – 异戊醇混合物

BioUltra, Molecular Biology, 49:1, ≥99.5% (chloroform + isoamyl alcohol, GC)

别名:

Chloroform:Isoamyl Alcohol Solvent Mix

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关于此项目

NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12352401
MDL number:
Assay:
≥99.5% (chloroform + isoamyl alcohol, GC)
Grade:
Molecular Biology
Technique(s):
GC/GC: suitable
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grade

Molecular Biology

Quality Level

product line

BioUltra

assay

≥99.5% (chloroform + isoamyl alcohol, GC)

form

liquid

technique(s)

GC/GC: suitable

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.001% free alkali (as NH3)

cation traces

Al: ≤0.5 mg/kg, Ba: ≤0.1 mg/kg, Bi: ≤0.1 mg/kg, Ca: ≤0.5 mg/kg, Cd: ≤0.05 mg/kg, Co: ≤0.02 mg/kg, Cr: ≤0.02 mg/kg, Cu: ≤0.02 mg/kg, Fe: ≤0.1 mg/kg, K: ≤0.5 mg/kg, Li: ≤0.1 mg/kg, Mg: ≤0.1 mg/kg, Mn: ≤0.02 mg/kg, Mo: ≤0.1 mg/kg, Na: ≤0.5 mg/kg, Ni: ≤0.02 mg/kg, Pb: ≤0.1 mg/kg, Sr: ≤0.1 mg/kg, Zn: ≤0.1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.1, λ: 280 nm Amax: 0.07

SMILES string

ClC(Cl)Cl.CC(C)CCO

InChI

1S/C5H12O.CHCl3/c1-5(2)3-4-6;2-1(3)4/h5-6H,3-4H2,1-2H3;1H

InChI key

BKHZIBWEHPHYAI-UHFFFAOYSA-N

Application

Chloroform – isoamyl alcohol mixture is a deproteinizing reagent used in conjunction with phenol for improving the efficiency of RNA and DNA (nucleic acid) extractions.

Other Notes

氯仿与异戊醇按 49:1 (v/v) 比率的混合物


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pictograms

Skull and crossbonesHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3

target_organs

Central nervous system, Liver,Kidney

存储类别

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

法规信息

易制毒化学品(2类)
危险化学品

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分析证书(COA)

Lot/Batch Number

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Purification of RNA by SDS solubilization and phenol extraction.
Rio DC, Ares M Jr, Hannon GJ, Nilsen TW.
Cold Spring Harbor Protocols, 2010, 5439-5439 (2010)
D.M. Wallace
Methods in Enzymology, 152, 35-35 (1987)
Bacterial RNA isolation.
Ares M.
Cold Spring Harbor Protocols, 1012, 1024-1027 (2012)



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