一般描述
Sepharose 2B是一种久经考验的交联琼脂糖凝胶过滤基质,常用于将亲和配体与基质的偶联。基质未经过预先激活,用户可以完成偶联中的所有步骤。
应用
Sepharose™ 2B已用于制备病毒体和血小板。
法律信息
Sepharose is a trademark of Cytiva
警示用语:
Danger
危险声明
危险分类
Flam. Liq. 2
储存分类代码
3 - Flammable liquids
WGK
WGK 3
Depletion of glycoprotein gp85 from virosomes made with Epstein-Barr virus proteins abolishes their ability to fuse with virus receptor-bearing cells.
Haddad RS and Hutt-Fletcher LM
Journal of Virology, 63, 4998-4998 (1989)
Regulation of the phosphoinositide cycle by Na+/H+ exchange and intracellular pH in human platelets.
G Luzzatto et al.
Biochimica et biophysica acta, 1084(1), 78-86 (1991-06-19)
We have found that thrombin-induced activation of protein kinase C (PKC) in platelets, measured by phosphorylation of the 47 kDa protein, is synergistically enhanced by the amiloride analogue ethylisopropylamiloride (EIA), a specific inhibitor of Na+/H+ exchange. This EIA effect was
Balakrishnan Sivaraman et al.
Langmuir : the ACS journal of surfaces and colloids, 28(5), 2745-2752 (2011-12-24)
Recent studies have shown that platelets can adhere to adsorbed albumin (Alb) through a receptor-mediated mechanism, but only if the Alb undergoes more than a critical degree of adsorption-induced unfolding. The objectives of this research were to investigate whether Alb
M H Kroll et al.
Biochimica et biophysica acta, 970(1), 61-67 (1988-06-08)
Experiments were performed to elucidate the role of adenosine 3': 5'-cyclic monophosphate (cAMP) in the control of platelet protein kinase C (PKC) activation. Platelet aggregation and secretion in response to 4 beta-phorbol 12-myristate 13-acetate (PMA) or 1-oleoyl-2-acetylglycerol (OAG) were inhibited
A Sen et al.
Cell, 17(2), 347-356 (1979-06-01)
A low molecular weight (LMW) protein phosphokinase enzyme that binds to actin has been isolated from murine sarcoma virions; this kinase activity is not present in nontransforming murine leukemia viruses. Sephadex G-75 gel filtration and affinity chromatography on actin-Sepharose conjugates
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