31174
Deoxyribonucleic acid, Plasmid (pBR 322 Plasmid) from E. coli RRI
≥80% supercoiled DNA basis
别名:
pBR322 Plasmid DNA from E. coli RRI
grade
Molecular Biology
assay
≥80% supercoiled DNA basis
mol wt
2.9 MDa, 4.363 bp, Mr ~2900000
absorbance ratio
A260/280 nm 1.8-1.9
shipped in
wet ice
storage temp.
−20°C
Biochem/physiol Actions
Unique Sites: Within the gene for ampicillin resistance: Pst I, Pvu I, Sca I; Within the gene for tetracycline resistance: BamH I, BspM I, EcoR V, Nhe I, Nru I, Sal I, Sph I, Xma III; other unique sites: Aat II, Ava I, Bal I, Bsm I, BspM II, Cla I, EcoR I, Hind III, Nde I, Pvu II, Ssp I, Sty I, Tth111 I.
One of the most commonly used cloning vectors, pBR322, confers resistance to ampicillin and tetracycline. The DNA sequence of the entire plasmid has been published.
Analysis Note
package quantity >5 A260 U/vial
Other Notes
Accession numbers: J01749 and V01119
One of the most commonly used cloning vectors
法规信息
新产品
此项目有
Complete nucleotide sequence of the Escherichia coli plasmid pBR322.
J G Sutcliffe
Cold Spring Harbor symposia on quantitative biology, 43 Pt 1, 77-90 (1979-01-01)
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