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Merck
CN

35847

2′,7′-二氯荧光素二乙酸酯

≥90% (HPLC)

别名:

2′,7′-二氯二氢荧光素二乙酸酯

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关于此项目

经验公式（希尔记法）:
C24H16Cl2O7
化学文摘社编号:
4091-99-0
分子量:
487.29
PubChem Substance ID:
24861977
UNSPSC Code:
12352200
Beilstein/REAXYS Number:
4610273
MDL number:
MFCD00128955

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SMILES string

CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C(c2cc1Cl)c4ccccc4C(O)=O

InChI

1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)

InChI key

PXEZTIWVRVSYOK-UHFFFAOYSA-N

assay

≥90% (HPLC)

mp

209-210 °C (lit.)

solubility

DMF: soluble, DMSO: soluble

fluorescence

λex 504 nm; λem 529 nm in 0.1 M Tris pH 8.0, esterase; H2O2

suitability

suitable for fluorescence

storage temp.

−20°C

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Cell-permeable fluorogenic probe used to measure reactive oxygen species (ROS) within cells by detection of enzymatically formed hydrogen peroxide

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W Jakubowski et al.
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Intracellular 2,7-dichlorofluorescin (H(2)DCF) oxidation is often used to measure the production of reactive oxygen species (ROS) within cells. The rate of H(2)DCF oxidation depends on the concentration of glutathione, which is an alternative target for ROS. Our results suggest that
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A Sanchez Ferrer et al.
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A sensitive enzymatic assay for oxidase reactions both in aqueous solution and in hexadecyltrimethylammoniumbromide (CTAB) reversed micelles has been developed. The assay is based on the fluorescence detection of dichlorofluorescein, which is formed by hydrogen peroxide oxidation of the nonfluorescent
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