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37008

毛地黄皂苷

~50% (TLC)

别名:

Digitin

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关于此项目

经验公式（希尔记法）:
C56H92O29
化学文摘社编号:
11024-24-1
分子量:
1229.31
EC Number:
234-255-6
UNSPSC Code:
12352200
MDL number:
MFCD00077729
Beilstein/REAXYS Number:
78654
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description

non-ionic

optical activity

[α]20/D −54.5±3°, c = 1% in methanol (dry matter)

mol wt

micellar avg mol wt 70,000

concentration

~50% (TLC)

aggregation number

60

CMC

<0.5 mM (20-25°C)

mp

230-240 °C (dec.) (lit.)

solubility

ethanol: 10 mg/mL, clear, almost colorless (hot)

SMILES string

C[C@@]12[C@]([C@@H]3C)([H])[C@](O[C@]34CC[C@@H](C)CO4)([H])[C@@H](O)[C@@]1([H])[C@@](CC[C@]5([H])[C@@]6(C[C@@H](O)[C@H](O[C@]([C@@H]([C@@H](O)[C@H]7O[C@@](O[C@H](CO)[C@@H](O)[C@@H]8O[C@@](OC[C@@H](O)[C@@H]9O)([H])[C@@H]9O)([H])[C@@H]8O[C@@](O[C@H](CO)[C@H

InChI

1S/C56H92O29/c1-19-7-10-56(75-17-19)20(2)31-45(85-56)37(67)32-22-6-5-21-11-26(24(61)12-55(21,4)23(22)8-9-54(31,32)3)76-50-42(72)39(69)44(30(16-60)80-50)81-53-48(47(36(66)29(15-59)79-53)83-49-40(70)33(63)25(62)18-74-49)84-52-43(73)46(35(65)28(14-58)78-52)82-51-41(71)38(68)34(64)27(13-57)77-51/h19-53,57-73H,5-18H2,1-4H3/t19-,20+,21+,22-,23+,24-,25-,26-,27-,28-,29-,30-,31+,32-,33+,34-,35+,36-,37+,38+,39-,40-,41-,42-,43-,44+,45-,46+,47+,48-,49+,50-,51+,52+,53+,54-,55+,56-/m1/s1

InChI key

UVYVLBIGDKGWPX-KUAJCENISA-N

Application

suitable for determination of cholesterol

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Permeabilizes plasma membranes of eukaryotic cells by complexing with membrane cholesterol and other unconjugated β-hydroxysterols. Use of digitonin-permeabilized adipocytes for cAMP studies; Selective precipitation of cholesterol; Solubilization of membrane proteins; Opioid receptor proteins.

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Solubilization of functional membrane proteins.
L M Hjelmeland et al.
Methods in enzymology, 104, 305-318 (1984-01-01)
T J Scallen et al.
The Journal of cell biology, 40(3), 802-813 (1969-03-01)
A major methodological problem in the intracellular localization of cholesterol is the nearly complete extraction of sterols during routine dehydration and embedding procedures for electron microscopy. Cholesterol digitonide (a sterol complex with digitonin), however, is qualitatively insoluble in these solvents.
Use of digitonin-permeabilized adipocytes for cAMP studies.
R A Mooney
Methods in enzymology, 159, 193-202 (1988-01-01)
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