39145
N-(7-二甲氨基-4-甲基-3-香豆素基)马来酰亚胺
≥99.0% (HPLC)
别名:
DACM-3
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关于此项目
经验公式（希尔记法）:
C16H14N2O4
化学文摘社编号:
分子量:
298.29
EC Number:
259-499-0
UNSPSC Code:
12352200
PubChem Substance ID:
Beilstein/REAXYS Number:
1545346
MDL number:
InChI key
ADEORFBTPGKHRP-UHFFFAOYSA-N
InChI
1S/C16H14N2O4/c1-9-11-5-4-10(17(2)3)8-12(11)22-16(21)15(9)18-13(19)6-7-14(18)20/h4-8H,1-3H3
SMILES string
CN(C)c1ccc2C(C)=C(N3C(=O)C=CC3=O)C(=O)Oc2c1
assay
≥99.0% (HPLC)
mp
218-220 °C (lit.)
fluorescence
λex 398 nm; λem 482 nm in 0.1 M phosphate pH 7.0 (after derivatization with 2-mercaptoethanol)
suitability
suitable for fluorescence
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Other Notes
Fluorescent reagent with high selectivity for protein thiols and with superior fluorescence and water solubility properties than dansyl chloride
法规信息
新产品
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Y. Kanaoka
Angewandte Chemie (International Edition in English), 89, 141-141 (1977)
M Hatanaka et al.
Biochimica et biophysica acta, 1209(1), 117-122 (1994-11-16)
Purified C9 with expected hemolytic and polymerizing activities was found to contain approximately 0.2 mol of sulfhydryl groups/mol of C9. By proteolysis of C9 with labeled SH groups, the SH residues on intact C9 were mapped to Cys-359 and Cys-384
L A Jurado et al.
Biochimica et biophysica acta, 1292(1), 188-196 (1996-01-04)
We studied the transition metal ion requirements for activity and sulfhydryl group reactivity in phospho enol pyruvate carboxykinase (PEP-carboxykinase; ATP:oxaloacetate carboxylase (transphosphorylating), EC 4.1.1.49), a key enzyme in the energy metabolism of the protozan parasite Trypanosoma (Schizotrypanum) cruzi. As for
T Ohyashiki et al.
Journal of biochemistry, 115(2), 224-229 (1994-02-01)
The effects of lipid peroxidation on the SH reactivity of the proteins in porcine intestinal brush-border membranes were examined using a fluorogenic thiol reagent, N-[7-dimethyl-amino-4-methylcoumarinyl]maleimide (DACM) in relation to lipid organization. Changes in the lipid organization were assessed by measurement
Kuikui Wang et al.
Talanta, 84(2), 400-405 (2011-03-08)
A sensitive and selective method for the paraoxon detection based on enzyme inhibition and fluorescence quenching was presented in this study. Under the catalytic effect of acetylcholinesterase (AChE), acetylthiocholine (ATCh) hydrolysis released thiocholine (TCh) which could react with N-(7-dimethylamino-4-methylcoumarin-3-yl) maleimide
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