technique(s)
affinity chromatography: suitable
storage temp.
2-8°C
Other Notes
Lysine is attached through the α-amino group to Sepharose™ 4B; the carboxy and ε-amino groups are free
Separation of the 4 molecular forms of human plasminogen; Binding of tissue-type plasminogen activator to lysine, lysine analogues and fibrin fragments
Legal Information
Lysine-Sepharose is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
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W Nieuwenhuizen et al.
Thrombosis and haemostasis, 61(2), 208-210 (1989-04-25)
At least four molecular forms of plasminogen are known. Two of those forms have glutamic acid at their amino-terminal end, and are designated as glu-plasminogen. The other two have lysine, methionine and/or valine as amino-terminal amino acid and are collectively
G A de Munk et al.
Biochemistry, 28(18), 7318-7325 (1989-09-05)
Human tissue-type plasminogen activator (t-PA) consists of five domains designated (starting from the N-terminus) finger, growth factor, kringle 1, kringle 2, and protease. The binding of t-PA to lysine-Sepharose and aminohexyl-Sepharose was found to require kringle 2. The affinity for