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Merck
CN

74030

氯化四唑氮蓝

≥88% (T)

别名:

氮四唑蓝, 氯化硝基四氮唑蓝, NBT, Nitro BT, 硝基蓝四氮唑, 硝基蓝四氮唑

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关于此项目

线性分子式:
C40H30N10O6 · 2Cl
化学文摘社编号:
298-83-9
分子量:
817.64
EC Number:
206-067-4
UNSPSC Code:
12171500
PubChem Substance ID:
329765398
Beilstein/REAXYS Number:
4115923
MDL number:
MFCD00012159

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InChI key

FSVCQIDHPKZJSO-UHFFFAOYSA-L

InChI

1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2

SMILES string

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8

assay

≥88% (T)

form

solid

impurities

≤10% solvent (mainly ethanol or methanol)

solubility

methanol: 50 mg/mL, clear, very strongly yellow

storage temp.

2-8°C

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Other Notes

Enzyme substrate e.g. for the determination of glucose-6-phosphate dehydrogenase; Precipitating agent for fibrinogen

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

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分析证书(COA)

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Isolation of human fibrinogen using nitroblue tetrazolium (NBT).
V Vila et al.
Clinica chimica acta; international journal of clinical chemistry, 138(2), 215-219 (1984-04-13)
D S Negi et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 25(2), 149-154 (1977-02-01)
An improved procedure is reported for the histochemical localization of glucose-6-phosphate dehydrogenase in fresh-frozen sections of animal and plant tissues. Used in this procedure are an incubation medium and an inert high-molecular-weight polyvinyl alcohol as a stabilizer to check enzyme
Pablo Gómez-Ochoa et al.
Veterinary immunology and immunopathology, 146(1), 97-99 (2012-03-01)
The nitroblue tetrazolium reduction test (NBT) is an assay based on the activation percentage of neutrophils in peripheral blood, that has been proposed for the follow up of canine leishmaniosis owing to the narrow relationship between the molecules involved in
N I Vera-Jimenez et al.
Fish & shellfish immunology, 34(5), 1216-1222 (2013-03-05)
The respiratory burst is an important feature of the immune system. The increase in cellular oxygen uptake that marks the initiation of the respiratory burst is followed by the production of reactive oxygen species (ROS) such as superoxide anion and
Jaime M Ross
Journal of visualized experiments : JoVE, (57)(57), e3266-e3266 (2011-12-07)
Mitochondrial DNA (mtDNA) defects are an important cause of disease and may underlie aging and aging-related alterations (1,2). The mitochondrial theory of aging suggests a role for mtDNA mutations, which can alter bioenergetics homeostasis and cellular function, in the aging
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