Acetate buffer concentrate pH 4.5

Dissolution Media Concentrates have been formulated to allow quick and easy dilution to a working solution. The label on each bottle indicates the volume of concentrate in the bottle and the volume to which it must be diluted to provide a final media solution that meets the applicable pharmacopoeia specifications. The user friendly format is designed so that each bottle is a single use unit. The instructions below illustrate just how easy it is to prepare the media.

1. Prepare a clean, dry mixing vessel of a suitable size, 6L, 10L or 25L.
2. Add purified water to approximately one third of the required volume i.e. 2L, 3L or 8L.
3. Carefully pour the entire contents of the bottle of concentrate into the vessel.
4. Rinse the bottle with purified water and add rinsing′s to the mixing vessel.
5. Add purified water to the required volume.
6. Mix thoroughly.
7. Measure the pH and proceed if within specification.
8. Adjust pH if necessary.

Dilution to target volume (10 L) results in ~2.99 g/L Sodium acetate and ~1.68 g/L acetic acid (14 mL of a 2M acetic acid).