83205
焦磷酸酶，无机 来源于面包酵母（酿酒酵母）
powder, off-white, ~70 U/mg
别名:
无机焦磷酸酶, 焦磷酸盐磷酸水解酶
form
powder
specific activity
~70 U/mg
mol wt
Mr ~71000
color
off-white
storage temp.
−20°C
General description
分子量：71kDa（由分子量为 32-35kDa 的两个相同亚基组成的同型二聚体）
吸光系数：E1% = 14.5 (280nm)
pI：4.75
这种普遍存在的酶可驱动生成焦磷酸盐的代谢反应，因为这些反应的平衡常数通常接近整数。催化的机理在文献中有所记述。无机焦磷酸酶是一种金属蛋白酶，达到最大活性需要 Mg2+ 的参与。虽然无机焦磷酸盐的水解反应在 Mg2+ 存在下具有特异性，但是如果存在锌 ADP 和 ATP 均会被水解。
吸光系数：E1% = 14.5 (280nm)
pI：4.75
这种普遍存在的酶可驱动生成焦磷酸盐的代谢反应，因为这些反应的平衡常数通常接近整数。催化的机理在文献中有所记述。无机焦磷酸酶是一种金属蛋白酶，达到最大活性需要 Mg2+ 的参与。虽然无机焦磷酸盐的水解反应在 Mg2+ 存在下具有特异性，但是如果存在锌 ADP 和 ATP 均会被水解。
Application
无机焦磷酸酶 (PPase) 是一种普遍存在的酶，可催化 PPi + H2O → 2Pi。
它在蛋白质、RNA 和 DNA 合成中发挥着重要作用。
它在蛋白质、RNA 和 DNA 合成中发挥着重要作用。
Other Notes
1 U corresponds to the amount of enzyme which will liberate 1 μmol of phosphate from inorganic pyrophosphate per minute at pH 7.2 and 25 °C
Mechanism of action
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
The mechanism of action of yeast inorganic pyrophosphatase.
B S Cooperman
Methods in enzymology, 87, 526-548 (1982-01-01)
Genetic manipulation of a "vacuolar" H(+)-PPase: from salt tolerance to yield enhancement under phosphorus-deficient soils.
Roberto A Gaxiola et al.
Plant physiology, 159(1), 3-11 (2012-03-22)
Zh I Rybchenko et al.
Ukrains'kyi biokhimichnyi zhurnal (1999 ), 84(3), 88-94 (2012-08-07)
The function of two electrogenic H+ -pumps in plant vacuolar membrane (tonoplast) and their response to the salt stress conditions and adaptogenic preparations have been studied. Experiments were carried out on tonoplast fraction isolated from the roots of corn seedlings
Ali Ferjani et al.
Plant signaling & behavior, 7(1), 38-42 (2012-02-04)
The synthesis of DNA, RNA, and de novo proteins is fundamental for early development of the seedling after germination, but such processes release pyrophosphate (PPi) as a byproduct of ATP hydrolysis. The over-accumulation of the inhibitory metabolite PPi in the
Habib Khoudi et al.
Journal of plant physiology, 169(1), 98-103 (2011-11-08)
Cadmium (Cd) is considered an extremely significant pollutant due to its high toxicity to many organisms. Plants have evolved several mechanisms to cope with Cd, the most important of which is vacuolar sequestration. Cadmium can be directly transported into vacuoles
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