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Merck
CN

93745

TSC 琼脂

NutriSelect® Basic, according to ISO, powder, selective and differential for Clostridium perfringens, pack of 500 g, suitable for microbiology

别名:

胰蛋白示-亚硫酸盐-环丝氨酸琼脂

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关于此项目

NACRES:
NA.74
UNSPSC Code:
41171606
Manufacturer/tradename:
NutriSelect® Basic
Agency:
according to ISO 15213:2003, according to ISO 7937:2004, according to ISO
Form:
powder
Suitability:
selective and differential for Clostridium perfringens
Application(s):
agriculture
clinical testing
environmental
food and beverages
pathogen testing
water monitoring, microbiology

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agency

according to ISO 15213:2003, according to ISO 7937:2004, according to ISO

sterility

non-sterile

form

powder

Quality Level

200

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L , ammonium ferric citrate, 1 g/L , meat peptone (peptic), 15 g/L , sodium disulfite, 1 g/L , soya peptone, 5 g/L , yeast extract, 5 g/L

packaging

pack of 500 g

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

final pH

7.6±0.2 (25 °C)

application(s)

agriculture
clinical testing
environmental
food and beverages
pathogen testing
water monitoring, microbiology

suitability

selective and differential for Clostridium perfringens

Application

用于从食物、临床标本和其它材料中分离和计数产气荚膜梭菌的营养形态和孢子（根据哈蒙等（1971））。

Preparation Note

基础琼脂：
取42 g溶于1L蒸馏水中。在121°C高压灭菌15分钟。冷却至约50°C。

TSC 琼脂：
向1升的基础琼脂中加入400 mg复水的D-环丝氨酸（货号 30020）作为无菌过滤的水溶液或2管产气荚膜梭菌T.S.C.补剂（货号P9352）和50 ml卵黄乳液（货号 17148）。充分混匀并倒至培养平板中。

无卵黄TSC琼脂（也用作琼脂覆层）：
加入400 mg复水的D-环丝氨酸（货号 30020）或2管产气荚膜梭菌T.S.C.补剂（货号P9352）至1升的基础琼脂，并充分混匀。

TSC 荧光琼脂：
向1升的基础琼脂中加入5.5 g复水的TSC琼脂补剂（货号80548）作为无菌过滤的水溶液。充分混匀并倒至平板中。

SFP 琼脂（Shahidi-Ferguson Perfringens 琼脂）：
向1升基础琼脂中加入2管产气荚膜梭菌S.F.P.补剂（货号P9477）和50 ml卵黄乳液（货号17148）。充分混匀并倒至无菌培养平板中。

Other Notes

我们现提供两种类型的培养基：GranuCult®优质颗粒状培养基和NutriSelect®高性价比粉末状培养基，满足用户不同层次的需求。
商标后的品级称号basic（基础）、plus（加强）或prime（特级）代表产品的不同质控水平，从基础的质控到标准质控加强版到全面合规的特级质控水平。
牡蛎中亚硫酸盐还原梭菌孢子和 D 群粪便链球菌的分离和计数；水中粪便梭状芽胞杆菌和产气荚膜梭菌的膜过滤计数

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

存储类别

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCN3789
BCCL5172
BCCM2837
BCCJ9304
BCCH6839

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S M Harmon et al.
Applied microbiology, 21(5), 922-927 (1971-05-01)
For the enumeration of viable vegetative cells and spores of Clostridium perfringens, noncommercial (laboratory prepared) sulfite-polymyxin-sulfadiazine (SPS) agar, tryptone-sulfite-neomycin (TSN) agar, and Shahidi-Ferguson-perfringens (SFP) agar were statistically compared to SPS agar without antibiotics. The selectivities of these four media were
D.P. Sartory
Water Research, 20, 1255-1255 (1986)
T J Easterbrook et al.
The Journal of applied bacteriology, 62(5), 413-419 (1987-05-01)
A comparative study of methods to enumerate sulphite-reducing Clostridium spores and Group D faecal streptococci in oysters demonstrated that pour plate solid agar techniques gave higher counts than liquid broth most probable number procedures. Reinforced clostridial broth with supplements to

商品

Detection, Identification and Differentiation of Clostridium perfringens

Clostridium perfringens can cause contamination in undercooked or improperly sterilized canned foods and water. Learn to detect, identify, and differentiate this pathogen.

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