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Merck
CN

93745

TSC 琼脂

NutriSelect® Basic, according to ISO, powder, selective and differential for Clostridium perfringens, pack of 500 g, suitable for microbiology

别名:

胰蛋白示-亚硫酸盐-环丝氨酸琼脂

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关于此项目

NACRES:
NA.74
UNSPSC Code:
41171606
Manufacturer/tradename:
NutriSelect® Basic
Agency:
according to ISO 15213:2003, according to ISO 7937:2004, according to ISO
Form:
powder
Suitability:
selective and differential for Clostridium perfringens
Application(s):
agriculture
clinical testing
environmental
food and beverages
pathogen testing
water monitoring, microbiology
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Quality Level

agency

according to ISO 15213:2003, according to ISO 7937:2004, according to ISO

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L , ammonium ferric citrate, 1 g/L , meat peptone (peptic), 15 g/L , sodium disulfite, 1 g/L , soya peptone, 5 g/L , yeast extract, 5 g/L

packaging

pack of 500 g

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

final pH

7.6±0.2 (25 °C)

application(s)

agriculture
clinical testing
environmental
food and beverages
pathogen testing
water monitoring, microbiology

suitability

selective and differential for Clostridium perfringens

Application

用于从食物、临床标本和其它材料中分离和计数产气荚膜梭菌的营养形态和孢子(根据哈蒙等(1971))。

Preparation Note

基础琼脂:
取42 g溶于1L蒸馏水中。在121°C高压灭菌15分钟。冷却至约50°C。

TSC 琼脂:
向1升的基础琼脂中加入400 mg复水的D-环丝氨酸(货号 30020)作为无菌过滤的水溶液或2管产气荚膜梭菌T.S.C.补剂(货号P9352)和50 ml卵黄乳液(货号 17148)。充分混匀并倒至培养平板中。

无卵黄TSC琼脂(也用作琼脂覆层):
加入400 mg复水的D-环丝氨酸(货号 30020)或2管产气荚膜梭菌T.S.C.补剂(货号P9352)至1升的基础琼脂,并充分混匀。

TSC 荧光琼脂:
向1升的基础琼脂中加入5.5 g复水的TSC琼脂补剂(货号80548)作为无菌过滤的水溶液。充分混匀并倒至平板中。

SFP 琼脂(Shahidi-Ferguson Perfringens 琼脂):
向1升基础琼脂中加入2管产气荚膜梭菌S.F.P.补剂(货号P9477)和50 ml卵黄乳液(货号17148)。充分混匀并倒至无菌培养平板中。

Other Notes

牡蛎中亚硫酸盐还原梭菌孢子和 D 群粪便链球菌的分离和计数;水中粪便梭状芽胞杆菌和产气荚膜梭菌的膜过滤计数
我们现提供两种类型的培养基:GranuCult®优质颗粒状培养基和NutriSelect®高性价比粉末状培养基,满足用户不同层次的需求。
商标后的品级称号basic(基础)、plus(加强)或prime(特级)代表产品的不同质控水平,从基础的质控到标准质控加强版到全面合规的特级质控水平。

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany


pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

存储类别

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

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商品

Clostridium perfringens can cause contamination in undercooked or improperly sterilized canned foods and water. Learn to detect, identify, and differentiate this pathogen.


S M Harmon et al.
Applied microbiology, 21(5), 922-927 (1971-05-01)
For the enumeration of viable vegetative cells and spores of Clostridium perfringens, noncommercial (laboratory prepared) sulfite-polymyxin-sulfadiazine (SPS) agar, tryptone-sulfite-neomycin (TSN) agar, and Shahidi-Ferguson-perfringens (SFP) agar were statistically compared to SPS agar without antibiotics. The selectivities of these four media were
D.P. Sartory
Water Research, 20, 1255-1255 (1986)
T J Easterbrook et al.
The Journal of applied bacteriology, 62(5), 413-419 (1987-05-01)
A comparative study of methods to enumerate sulphite-reducing Clostridium spores and Group D faecal streptococci in oysters demonstrated that pour plate solid agar techniques gave higher counts than liquid broth most probable number procedures. Reinforced clostridial broth with supplements to



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货号GTIN
93745-500G04061833283134