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NIST8671

NISTmAb, 人源化IgG1k单克隆抗体

NIST® SRM® 8671

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NACRES:
NA.41
UNSPSC Code:
41116107
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antibody product type

primary antibodies

grade

certified reference material

form

liquid

manufacturer/tradename

NIST®

technique(s)

UV/Vis spectroscopy: suitable, liquid chromatography (LC): suitable, mass spectrometry (MS): suitable

format

matrix material

General description

NISTmAb，人源化IgG1k 单克隆抗体，是一种在鼠悬液培养物中表达的重组抗体。它是具有两个相同的轻链和两个相同的重链的同型二聚体，它们通过链间和链内二硫键进行连接，分子量为150 kDa。该蛋白质具有低丰度的翻译后修饰，包括蛋氨酸氧化、脱酰胺和糖基化。该人源化单克隆抗体还具有N末端焦谷氨酸、C末端赖氨酸剪切和重链糖基化。

一单位RM 8671 由一个含800 µL 10 mg/mL NIST IgG1κ单克隆抗体（NISTmAb）、12.5 mmol/L L-组氨酸和L-组氨酸盐酸盐（pH 6.0）的冷冻管组成。

SRM 8671_Cert

SRM 8671 _SDS

Application

NISTmAb，人源化IgG1k 单克隆抗体已作为标准品，用于在自底向上质谱中对 从头 测序算法进行测试。本标准物质（RM）主要用于评估单克隆抗体生理化学和生物物理属性测定方法的效果。它还为开发治疗性蛋白质表征的新技术提供了代表性的测试分子。其他潜在用途包括：
  • 系统适用性测试、
  • 方法建立或仪器性能和可变性测定、
  • 比较变化的分析测试方法以及
  • 协助方法鉴定。

Analysis Note

NIST8671供研究使用。不适用于动物或人类食用、临床测试或治疗用途。

Other Notes

  • RM 8671用于研究用途。
  • 有关失效日期、储存、安全性、使用和来源的详细信息见于NIST 证书。 
  • 有关生物材料、处置和运输的信息见于SDS。

Legal Information

NIST is a registered trademark of National Institute of Standards and Technology
SRM is a registered trademark of National Institute of Standards and Technology

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存储类别

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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商品

Middle-Up Antibody Analysis by RP Chromatography

Compare columns in resolving medium-sized antibody fragments after digestion with DTT or IdeS using Reversed-Phase Chromatography for analysis.

Charge Variant Analysis (CVA) with Strong Cation Exchange

Learn about the separation of several charge variants of two monoclonal antibodies, NISTmAb and Infliximab, using a polymeric cation exchange column and MS-compatible mobile phases.

An Optimized Protocol for Peptide Mapping of Therapeutic Monoclonal Antibodies with Minimum Deamidation and Oxidation Artifacts

An optimized peptide mapping protocol using NISTmAb as a model monoclonal antibody, shorter incubation times, and improved digestion buffer to demonstrate minimal artificial asparagine deamidation and methionine oxidation.

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全球贸易项目编号

货号GTIN
NIST867104061838660374