C0537
5-羧基荧光素
≥99% purity (HPLC), powder
别名:
5-FAM
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关于此项目
经验公式(希尔记法):
C21H12O7
化学文摘社编号:
分子量:
376.32
Beilstein:
57037
MDL编号:
UNSPSC代码:
12171500
PubChem化学物质编号:
NACRES:
NA.47
产品名称
5-羧基荧光素, ≥99% (HPLC)
质量水平
方案
≥99% (HPLC)
表单
powder
溶解性
0.1 M NaOH: 9.80-10.20 mg/mL, clear to slightly hazy
应用
diagnostic assay manufacturing
hematology
histology
储存温度
2-8°C
SMILES字符串
OC(=O)c1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35
InChI
1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)
InChI key
NJYVEMPWNAYQQN-UHFFFAOYSA-N
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应用
5-羧基荧光素已被用作毛细管等速电泳中的一种内部标记,以量化基于电荷的低密度脂蛋白的亚组分。
生化/生理作用
5-羧基荧光素被用于细胞染色以区分活细胞和死细胞。它有助于监测细胞膜的整体性和完整性。它还被用于测量细胞内pH。5-羧基荧光素二乙酸酯是一种非荧光分子,在被细胞内酯酶裂解后可得到绿色荧光产物5-羧基荧光素。在活细胞中,荧光分子对细胞膜是不可渗透的。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
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